CD28 Antibody (3H1179): sc-70612

CD28 Antibody (3H1179) is a rat monoclonal IgG2b antibody that detects CD28 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-CD28 antibody (3H1179) is available as the non-conjugated format. CD28 protein plays a crucial role in T cell activation and survival, serving as a primary co-stimulatory receptor that enhances T cell responses upon engagement with CD80 and CD86 ligands found on antigen-presenting cells. This interaction is vital for effective immune responses, promoting T cell proliferation and enhancing lymphokine production, which are essential for orchestrating immune response. CD28 protein′s importance is underscored by its ability to influence T cell fate; while predominantly expressed on resting T cells, CD28 engagement leads to a cascade of signaling events critical for T cell activation. Additionally, CD28 presence on T cells allows for a more robust immune response, particularly in infections and tumor immunity, making CD28 a significant target for therapeutic interventions in various diseases. CD28 (3H1179) antibody is an essential tool for researchers studying T cell biology and immune regulation mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD28 Antibody (3H1179) References:

1. Down-regulation of CD28 expression by TNF-alpha.  |  Bryl, E., et al. 2001. J Immunol. 167: 3231-8. PMID: 11544310
2. CD4+CD28- costimulation-independent T cells in multiple sclerosis.  |  Markovic-Plese, S., et al. 2001. J Clin Invest. 108: 1185-94. PMID: 11602626
3. CD28 as a molecular amplifier extending TCR ligation and signaling capabilities.  |  Michel, F., et al. 2001. Immunity. 15: 935-45. PMID: 11754815
4. CD28 signal enhances apoptosis of CD8 T cells after strong TCR ligation.  |  Yu, XZ., et al. 2003. J Immunol. 170: 3002-6. PMID: 12626553
5. CD8+CD28-, suppressive T cells in systemic lupus erythematosus.  |  Tulunay, A., et al. 2008. Lupus. 17: 630-7. PMID: 18625635
6. CD28 and CD3 have complementary roles in T-cell traction forces.  |  Bashour, KT., et al. 2014. Proc Natl Acad Sci U S A. 111: 2241-6. PMID: 24469820
7. CD28 and 41BB Costimulation Enhances the Effector Function of CD19-Specific Engager T Cells.  |  Velasquez, MP., et al. 2017. Cancer Immunol Res. 5: 860-870. PMID: 28821531
8. TCR and CD28 Concomitant Stimulation Elicits a Distinctive Calcium Response in Naive T Cells.  |  Xia, F., et al. 2018. Front Immunol. 9: 2864. PMID: 30564247
9. The role of CD28 costimulation in the generation of cytotoxic T lymphocytes.  |  Azuma, M. and Lanier, LL. 1995. Curr Top Microbiol Immunol. 198: 59-74. PMID: 7774283
10. Immunosuppression through blockade of CD28:B7-mediated costimulatory signals.  |  Judge, TA., et al. 1996. Immunol Res. 15: 38-49. PMID: 8739564

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD28 Antibody (3H1179) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-CD28 Antibody (3H1179) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD28 Antibody (3H1179) Specifications

Alternate Product Name: TP44

Product Clone Name: 3H1179

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: CD28

Epitope Species: human

Product Reactivity/Detection: CD28

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for CD28 Antibody (3H1179): N/A

The preferred CD28 antibody is CD28 Antibody (CD28.2): sc-19655.