CD27 Double Nickase Plasmid (h): sc-401938-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD27 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD27 Double Nickase Plasmid (h) and CD27 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD27. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD27 Antibody (B-8): sc-25289
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD27 Double Nickase Plasmid (h) | sc-401938-NIC | 20 µg | $410.00 | |||
CD27 Double Nickase Plasmid (h2) | sc-401938-NIC-2 | 20 µg | $410.00 |
CD27 is a TNF receptor superfamily costimulatory molecule expressed on activated T cells, memory B cells, and subsets of NK cells, where engagement by CD70 amplifies antigen-driven activation. CD27 signaling promotes lymphocyte survival, proliferation, and differentiation through TRAF-mediated pathways that converge on NF-κB and MAPK cascades, shaping cytokine production and adaptive immune memory. Dysregulated CD27–CD70 activity has been associated with altered immune homeostasis, chronic inflammation, and tumor-immune interactions, and inherited defects in CD27 can impair antiviral immunity and humoral responses. As a surface receptor with well-defined ligand biology, CD27 is frequently studied in mechanisms of immune activation, exhaustion, and lymphocyte lineage fate decisions.
CD27 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD27 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD27. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD27 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD27-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.