Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD24 Double Nickase Plasmid (m): sc-419540-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD24 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD24 Double Nickase Plasmid (m) and CD24 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Cd24a. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD24 Antibody (SN3): sc-19585
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD24 Double Nickase Plasmid (m) | sc-419540-NIC | 20 µg | $410.00 | |||
CD24 Double Nickase Plasmid (m2) | sc-419540-NIC-2 | 20 µg | $410.00 |
Cd24a encodes the glycosylphosphatidylinositol (GPI)-anchored surface protein CD24, a heavily glycosylated immunomodulatory molecule expressed on multiple hematopoietic and epithelial cell types in mouse. CD24 participates in cell–cell interactions and tuning of innate and adaptive immune responses, influencing leukocyte activation, adhesion dynamics, and differentiation programs. Through ligand-dependent signaling and association with membrane microdomains, CD24 can shape inflammatory signaling cascades and impact processes such as antigen responsiveness and tissue homeostasis. Dysregulated Cd24a expression has been linked to altered immune regulation and cellular plasticity in disease-relevant contexts, supporting its use as a molecular handle for studying inflammation-associated phenotypes and tumor-immune interactions in mouse model systems.
CD24 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Cd24a locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Cd24a. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Cd24a function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Cd24a-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.