Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD20 Double Nickase Plasmid (h): sc-416765-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD20 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD20 Double Nickase Plasmid (h) and CD20 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MS4A1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD20 Antibody (D-10): sc-393894
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD20 Double Nickase Plasmid (h) | sc-416765-NIC | 20 µg | $410.00 | |||
CD20 Double Nickase Plasmid (h2) | sc-416765-NIC-2 | 20 µg | $410.00 |
MS4A1 encodes CD20, a tetraspan membrane protein selectively expressed on B lymphocytes that contributes to B-cell activation, proliferation, and differentiation. CD20 is implicated in regulating Ca2+ flux and organizing membrane microdomains that coordinate B-cell receptor signaling and downstream pathways such as PI3K/AKT, NF-κB, and MAPK. Expression of CD20 defines key stages of B-cell development and is widely used as a lineage marker in immunology. Dysregulated B-cell signaling and altered MS4A1 expression are relevant to studies of autoimmune inflammation and B-cell–driven hematologic disease biology.
CD20 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MS4A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MS4A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MS4A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MS4A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.