CD20 Antibody (BC-1): sc-58983

CD20 Antibody (BC-1) is a mouse monoclonal IgM antibody that detects CD20 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-CD20 antibody (BC-1) targets CD20, a crucial leukocyte surface antigen characterized by four transmembrane regions and cytoplasmic N- and C-termini. The cytoplasmic domain of CD20 contains multiple phosphorylation sites, which can lead to the generation of different isoforms, thereby influencing functional diversity. CD20 is primarily expressed on B cells and has been identified on both normal and neoplastic T cells, highlighting relevance in various immune responses and potential roles in cancer biology. CD20 acts as a calcium-permeable cation channel, playing a significant role in cellular signaling by facilitating the transition from G0 to G1 phase of the cell cycle, particularly in response to insulin-like growth factor 1 (IGF-1). CD20 activation is mediated by the IGF-1 receptor through alpha subunits of heterotrimeric G proteins, leading to a marked increase in DNA synthesis, which involves basic helix-loop-helix leucine zipper transcription factors. This intricate regulation of CD20 underscores importance in both normal immune function and pathological conditions, making CD20 (BC-1) monoclonal antibody an essential tool for researchers studying B cell biology and related diseases.

The preferred CD20 antibody is CD20 Antibody (B-LY1): sc-19990.