CD16 Lentiviral Activation Particles (h): sc-400544-LAC
- Target species: human
- 200 µl of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particles
- CD16 Lentiviral Activation Particles (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells
- CD16 Lentiviral Activation Particles (h) contain the following SAM Activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nt guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genes
- Upon transduction, the SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by CD16 Lentiviral Activation Plasmid (h) and CD16 Lentiviral Activation Plasmid (h2) target distinct regulatory regions of the FCGR3A promoter. One or both designs may be available
- Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: CD16 Antibody (DJ130c): sc-20052
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD16 Lentiviral Activation Particles (h) | sc-400544-LAC | 200 µl | $455.00 | |||
CD16 Lentiviral Activation Particles (h2) | sc-400544-LAC-2 | 200 µl | $455.00 |
Human FCGR3A encodes CD16 (FcγRIIIa), a low-affinity Fc receptor for IgG expressed prominently on natural killer cells, macrophages, and subsets of monocytes. CD16 engagement by immune complexes initiates signaling through ITAM-bearing adaptor chains (FcRγ/CD3ζ), activating Src/Syk-dependent pathways that regulate antibody-dependent cellular cytotoxicity, phagocytosis, cytokine production, and modulation of innate immune thresholds. Variation in FCGR3A expression and receptor activity influences Fc-mediated effector functions and has been associated with immune dysregulation phenotypes studied in autoimmunity, chronic infection, and cancer immunobiology. These properties make CD16 a widely used marker and functional node for interrogating Fc receptor signaling, NK cell activation programs, and antibody–immune complex biology.
CD16 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient FCGR3A upregulation across a broader range of human cell types.
CD16 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the FCGR3A transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous CD16 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native FCGR3A genomic locus and regulatory architecture.
The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.