CD16-B Antibody (Z64): sc-73799

CD16-B Antibody (Z64) is a mouse monoclonal IgG2b antibody that detects CD16-B protein of human origin by western blotting (WB), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-CD16-B antibody (Z64) is available as the non-conjugated format. CD16-B protein, also known as Fcγ RIIIB, is a glycosylphosphatidylinositol-anchored receptor primarily expressed on neutrophils, playing a crucial role in the immune response by mediating antibody-dependent cellular cytotoxicity. CD16-B′s unique structure, which includes a glycosylphosphatidylinositol anchor, allows release from the cell surface upon activation, thus modulating immune responses and influencing inflammation. This dynamic function is particularly important in various diseases, including autoimmune disorders and infections, where neutrophil activity regulation is vital for effective immune defense. CD16-B is polymorphic, with two alleles designated NA1 and NA2, which can affect binding affinity and functional responses. Understanding CD16-B′s structure and function can provide insights into therapeutic strategies targeting immune modulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD16-B Antibody (Z64) References:

1. The membrane anchor influences ligand binding two-dimensional kinetic rates and three-dimensional affinity of FcgammaRIII (CD16).  |  Chesla, SE., et al. 2000. J Biol Chem. 275: 10235-46. PMID: 10744709
2. The PI-linked receptor FcRIII is released on stimulation of neutrophils.  |  Huizinga, TW., et al. 1988. Nature. 333: 667-9. PMID: 2967437
3. The Fc receptor for IgG on human natural killer cells: phenotypic, functional, and comparative studies with monoclonal antibodies.  |  Perussia, B., et al. 1984. J Immunol. 133: 180-9. PMID: 6233371
4. Human neutrophil Fc gamma receptor distribution and structure.  |  Fleit, HB., et al. 1982. Proc Natl Acad Sci U S A. 79: 3275-9. PMID: 6808506
5. Ligand binding and phagocytosis by CD16 (Fc gamma receptor III) isoforms. Phagocytic signaling by associated zeta and gamma subunits in Chinese hamster ovary cells.  |  Nagarajan, S., et al. 1995. J Biol Chem. 270: 25762-70. PMID: 7592758
6. A triallelic Fc gamma receptor type IIIA polymorphism influences the binding of human IgG by NK cell Fc gamma RIIIa.  |  de Haas, M., et al. 1996. J Immunol. 156: 2948-55. PMID: 8609432
7. The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding.  |  Tamm, A. and Schmidt, RE. 1996. J Immunol. 157: 1576-81. PMID: 8759741