CD15 Antibody (By87a): sc-53290

CD15 Antibody (By87a) is a mouse monoclonal IgM antibody that detects CD15 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD15, also known as Lewis X or LeX, is a carbohydrate antigen synthesized by FucT-IV (alpha 1,3-fucosyltransferase IV) and is primarily located on the surface of leukocytes and certain tumor cells. This trisaccharide structure is crucial for its function as an adhesion molecule, facilitating calcium-mediated homotypic binding among cells. CD15 on the cell surface promotes strong self-aggregation, which is essential for various physiological processes, including immune responses and inflammation. Furthermore, CD15 serves as a ligand for selectins, proteins that play a pivotal role in mediating leukocyte-specific interactions, thereby enhancing CD15′s importance in cell adhesion and migration during immune responses. CD15 (By87a) antibody is an invaluable tool for researchers studying cell adhesion mechanisms and the role of fucosylation in cellular interactions.

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CD15 Antibody (By87a) References:

1. Flow cytometric assessment of CD15+CD117+ cells for the detection of minimal residual disease in adult acute myeloid leukaemia.  |  Nakamura, K., et al. 2000. Br J Haematol. 108: 710-6. PMID: 10792273
2. CD15, CD24, and CD29 define a surface biomarker code for neural lineage differentiation of stem cells.  |  Pruszak, J., et al. 2009. Stem Cells. 27: 2928-40. PMID: 19725119
3. CD133, CD15/SSEA-1, CD34 or side populations do not resume tumor-initiating properties of long-term cultured cancer stem cells from human malignant glio-neuronal tumors.  |  Patru, C., et al. 2010. BMC Cancer. 10: 66. PMID: 20181261
4. Clonogenic CD15 immunoreactive radial glial cells from the developing human lateral ganglionic eminence.  |  Buttler, D., et al. 2013. Curr Pharm Biotechnol. 14: 29-35. PMID: 23092258
5. Retinoic acid induces mouse bone marrow-derived CD15⁺, Oct4⁺ and CXCR4⁺ stem cells into male germ-like cells in a two-dimensional cell culture system.  |  Kashani, IR., et al. 2014. Cell Biol Int. 38: 782-9. PMID: 24677291
6. TNF-α enhancement of CD62E mediates adhesion of non-small cell lung cancer cells to brain endothelium via CD15 in lung-brain metastasis.  |  Jassam, SA., et al. 2016. Neuro Oncol. 18: 679-90. PMID: 26472821
7. DNA methylation profiling reveals a pathological signature that contributes to transcriptional defects of CD34+ CD15- cells in early chronic-phase chronic myeloid leukemia.  |  Maupetit-Mehouas, S., et al. 2018. Mol Oncol. 12: 814-829. PMID: 29575763
8. The Comparison Between Microwave and Autoclave as Antigen Retrieval Methods for Immunohistochemical Detection of CD15 and CD30 in Hodgkin's Lymphoma.  |  Kosari, F. and Ghaffari, F. 2018. Iran J Pathol. 13: 390-396. PMID: 30774676
9. Increased expression of neutrophil CD15 correlates with the severity of anterior ocular surface damage in type II diabetes mellitus.  |  Zhmud, T., et al. 2024. Eur J Ophthalmol. 34: 1515-1520. PMID: 38153338
10. A carbohydrate structure associated with CD15 (Lewis x) on myeloid cells is a novel ligand for human CD2.  |  Warren, HS., et al. 1996. J Immunol. 156: 2866-73. PMID: 8609406

The preferred CD15 antibody is CD15 Antibody (C3D-1): sc-19648.