CD15 Antibody (B40.8): sc-517384

CD15 Antibody (B40.8) is a mouse monoclonal IgM antibody that detects CD15 in human samples through immunofluorescence (IF) and flow cytometry (FCM) applications. CD15, also known as Lewis X or LeX, is a carbohydrate antigen synthesized by the enzyme fucosyltransferase IV (FucT-IV), which catalyzes the addition of fucose to sugar acceptor molecules, resulting in the formation of a trisaccharide structure. CD15 is predominantly located on the surface of leukocytes and certain tumor cells, where CD15 plays a crucial role as an adhesion molecule. CD15 facilitates calcium-mediated homotypic binding, leading to strong self-aggregation of cells that express high levels of CD15 in the presence of calcium ions. Furthermore, CD15 serves as a ligand for selectins, which are essential for mediating leukocyte-specific interactions during immune responses. CD15′s ability to promote cell adhesion and aggregation underscores CD15′s importance in various physiological processes, including inflammation and immune cell trafficking, making CD15 a significant target for research in immunology and cancer biology. Anti-CD15 antibody (B40.8) is an invaluable tool for studying these interactions and the functional implications of CD15 in human health and disease.

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CD15 Antibody (B40.8) References:

1. The pattern of gene expression in human CD15+ myeloid progenitor cells.  |  Lee, S., et al. 2001. Proc Natl Acad Sci U S A. 98: 3340-5. PMID: 11248080
2. Coexpression of CD15 and CD20 by Reed-Sternberg cells in Hodgkin's disease.  |  Zukerberg, LR., et al. 1991. Am J Pathol. 139: 475-83. PMID: 1716042
3. The micro-RNA 199b-5p regulatory circuit involves Hes1, CD15, and epigenetic modifications in medulloblastoma.  |  Andolfo, I., et al. 2012. Neuro Oncol. 14: 596-612. PMID: 22411914
4. CD133/CD15 defines distinct cell subpopulations with differential in vitro clonogenic activity and stem cell-related gene expression profile in in vitro propagated glioblastoma multiforme-derived cell line with a PNET-like component.  |  Kahlert, UD., et al. 2012. Folia Neuropathol. 50: 357-68. PMID: 23319191
5. Characterization of human neutrophil glycoproteins expressing the CD15 differentiation antigen (3-fucosyl-N-acetyllactosamine).  |  Albrechtsen, M. and Kerr, MA. 1989. Br J Haematol. 72: 312-20. PMID: 2569888
6. Increased Neutrophil Count and Decreased Neutrophil CD15 Expression Correlate With TB Disease Severity and Treatment Response Irrespective of HIV Co-infection.  |  Ndlovu, LN., et al. 2020. Front Immunol. 11: 1872. PMID: 32983107
7. High expression of neutrophil and monocyte CD64 with simultaneous lack of upregulation of adhesion receptors CD11b, CD162, CD15, CD65 on neutrophils in severe COVID-19.  |  Karawajczyk, M., et al. 2021. Ther Adv Infect Dis. 8: 20499361211034065. PMID: 34377464
8. Localization of the CD15-epitope in the inner ear of the developing mouse.  |  Meyer zym Gottesberge, AM. and Mai, JK. 1996. Cell Tissue Res. 283: 395-401. PMID: 8593669
9. Expression of CD15 in normal and metaplastic Paneth cells of the digestive tract.  |  Ariza, A., et al. 1996. J Clin Pathol. 49: 474-7. PMID: 8763261
10. Developmental expression of the CD15 epitope in the hippocampus of the mouse.  |  Ashwell, KW. and Mai, JK. 1997. Cell Tissue Res. 289: 17-23. PMID: 9182597

The preferred CD15 antibody is CD15 Antibody (C3D-1): sc-19648.