Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD130/gp130 Double Nickase Plasmid (h): sc-400301-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD130/gp130 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD130/gp130 Double Nickase Plasmid (h) and CD130/gp130 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL6ST. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD130/gp130 Antibody (E-8): sc-376280
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD130/gp130 Double Nickase Plasmid (h) | sc-400301-NIC | 20 µg | $410.00 | |||
CD130/gp130 Double Nickase Plasmid (h2) | sc-400301-NIC-2 | 20 µg | $410.00 |
IL6ST encodes CD130/gp130, the shared signal-transducing subunit for the IL-6 family of cytokine receptors that couples ligand engagement to intracellular kinase cascades. Upon receptor complex formation, gp130 activates JAK kinases and downstream STAT3/STAT1, and can also engage MAPK/ERK and PI3K–AKT pathways to regulate transcriptional programs controlling inflammation, differentiation, survival, and acute-phase responses. As a central node in cytokine signaling, altered IL6ST-dependent signaling is associated with dysregulated immune homeostasis and has been studied in contexts including chronic inflammatory states and oncogenic STAT3 pathway activation. These properties make gp130 a frequent target for mechanistic studies of cytokine responsiveness, transcriptional rewiring, and pathway cross-talk in human cells.
CD130/gp130 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL6ST locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL6ST. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL6ST function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL6ST-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.