Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CCNB1/cyclin B1 Double Nickase Plasmid (h): sc-400114-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CCNB1/cyclin B1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CCNB1/cyclin B1 Double Nickase Plasmid (h) and CCNB1/cyclin B1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CCNB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CCNB1/cyclin B1 Antibody (GNS1): sc-245
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CCNB1/cyclin B1 Double Nickase Plasmid (h) | sc-400114-NIC | 20 µg | $410.00 | |||
CCNB1/cyclin B1 Double Nickase Plasmid (h2) | sc-400114-NIC-2 | 20 µg | $410.00 |
CCNB1 encodes cyclin B1, a core regulatory subunit of the CDK1 kinase complex that drives the G2/M transition and coordinates mitotic entry. Cyclin B1 accumulation and nuclear translocation promote processes including chromosome condensation, nuclear envelope breakdown, and spindle assembly through tightly timed phosphorylation cascades. CCNB1 activity is integrated with checkpoint signaling pathways such as ATR/CHK1 and the spindle assembly checkpoint to enforce genomic stability. Dysregulated CCNB1 expression or turnover is frequently associated with proliferative states and chromosomal instability, making it a widely used marker and mechanistic node in cell-cycle and cancer biology research.
CCNB1/cyclin B1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CCNB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CCNB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CCNB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CCNB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.