CCDC25 Double Nickase Plasmid (h): sc-408656-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CCDC25 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CCDC25 Double Nickase Plasmid (h) and CCDC25 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CCDC25. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CCDC25 Antibody (F-7): sc-515201
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CCDC25 Double Nickase Plasmid (h) | sc-408656-NIC | 20 µg | $410.00 | |||
CCDC25 Double Nickase Plasmid (h2) | sc-408656-NIC-2 | 20 µg | $410.00 |
Human CCDC25 encodes a coiled-coil domain–containing protein that is thought to contribute to protein–protein interaction scaffolding and spatial organization of signaling or structural complexes. Coiled-coil proteins frequently participate in cytoskeletal regulation, membrane-associated trafficking, and coordination of cell polarity, suggesting CCDC25 may influence cellular architecture and dynamic remodeling. Altered expression or perturbation of coiled-coil network components has been linked to dysregulated proliferation, migration, and stress responses, making CCDC25 a relevant target for mechanistic studies in cancer biology and related pathophysiology. Investigating CCDC25 function can help clarify how coiled-coil–mediated assemblies integrate signaling and structural pathways that shape cell behavior.
CCDC25 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CCDC25 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CCDC25. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CCDC25 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CCDC25-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.