CCDC155 Double Nickase Plasmid (h): sc-410329-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CCDC155 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CCDC155 Double Nickase Plasmid (h) and CCDC155 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CCDC155. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CCDC155 Double Nickase Plasmid (h) | sc-410329-NIC | 20 µg | $410.00 |
CCDC155 encodes a coiled-coil domain–containing protein predicted to function as a scaffold that supports protein–protein interactions and spatial organization of intracellular complexes. Coiled-coil proteins commonly participate in cytoskeletal dynamics, vesicle trafficking, centrosome-associated processes, and nuclear architecture, linking CCDC155 to fundamental mechanisms that coordinate cell structure and signaling. Although CCDC155 remains relatively under-characterized, perturbation of coiled-coil scaffolds can influence genome stability, cell-cycle progression, and stress-response pathways that are frequently altered in human disease. As a result, CCDC155 is of interest for dissecting context-dependent regulatory networks in cell biology and for mapping genotype–phenotype relationships in model systems.
CCDC155 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CCDC155 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CCDC155. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CCDC155 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CCDC155-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.