CCDC125 Double Nickase Plasmid (h): sc-415026-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CCDC125 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CCDC125 Double Nickase Plasmid (h) and CCDC125 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CCDC125. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CCDC125 Double Nickase Plasmid (h) | sc-415026-NIC | 20 µg | $410.00 | |||
CCDC125 Double Nickase Plasmid (h2) | sc-415026-NIC-2 | 20 µg | $410.00 |
CCDC125 encodes a coiled-coil domain–containing protein predicted to function as a scaffold for protein–protein interactions, supporting the organization of intracellular complexes and spatial regulation of signaling. Coiled-coil proteins commonly contribute to cytoskeletal architecture, vesicular transport, and coordination of cell-cycle and stress-response pathways, although the specific molecular partners of CCDC125 remain incompletely defined. Altered expression or dysregulated network connectivity of coiled-coil scaffolds is frequently linked to defects in genome maintenance, mitotic progression, and cellular homeostasis. These features make CCDC125 a relevant target for mechanistic studies connecting subcellular organization to phenotypes observed in proliferative and stress-associated disease contexts.
CCDC125 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CCDC125 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CCDC125. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CCDC125 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CCDC125-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.