Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CBS Double Nickase Plasmid (h): sc-400877-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CBS Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CBS Double Nickase Plasmid (h) and CBS Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CBS. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CBS Antibody (B-4): sc-133154
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CBS Double Nickase Plasmid (h) | sc-400877-NIC | 20 µg | $410.00 | |||
CBS Double Nickase Plasmid (h2) | sc-400877-NIC-2 | 20 µg | $410.00 |
Human CBS (cystathionine β-synthase) is a pyridoxal phosphate–dependent enzyme that catalyzes the first committed step of the transsulfuration pathway, converting homocysteine and serine to cystathionine and linking one-carbon metabolism to cysteine and glutathione biosynthesis. By regulating homocysteine clearance and redox buffering capacity, CBS influences methylation potential, oxidative stress responses, and broader sulfur amino acid metabolism. CBS activity also contributes to endogenous hydrogen sulfide production, a signaling molecule implicated in mitochondrial function and cellular stress adaptation. Dysregulation of CBS is associated with altered homocysteine homeostasis and has been studied in contexts including inborn errors of metabolism and neurovascular and inflammatory phenotypes relevant to basic research.
CBS Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CBS locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CBS. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CBS function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CBS-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.