CBARA1/MICU1 Double Nickase Plasmid (h): sc-418304-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CBARA1/MICU1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CBARA1/MICU1 Double Nickase Plasmid (h) and CBARA1/MICU1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MICU1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CBARA1/MICU1 Antibody (D-10): sc-518183
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CBARA1/MICU1 Double Nickase Plasmid (h) | sc-418304-NIC | 20 µg | $410.00 | |||
CBARA1/MICU1 Double Nickase Plasmid (h2) | sc-418304-NIC-2 | 20 µg | $410.00 |
MICU1 (CBARA1) encodes a Ca2+-binding regulatory subunit of the mitochondrial calcium uniporter complex that helps set the threshold for mitochondrial Ca2+ uptake in response to cytosolic calcium signals. By modulating mitochondrial Ca2+ homeostasis, MICU1 influences oxidative phosphorylation, reactive oxygen species balance, and coupling between Ca2+ signaling and metabolic output, with downstream effects on apoptosis and stress adaptation. Disrupted MICU1 function has been linked to neuromuscular and neurodevelopmental phenotypes, consistent with its role in maintaining mitochondrial bioenergetic stability in excitable tissues. MICU1 is therefore widely studied in pathways connecting mitochondrial signaling, cellular metabolism, and Ca2+-dependent transcriptional responses.
CBARA1/MICU1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MICU1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MICU1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MICU1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MICU1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.