caspase-8 Double Nickase Plasmid (h): sc-400147-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- caspase-8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- caspase-8 Double Nickase Plasmid (h) and caspase-8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CASP8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: caspase-8 Antibody (8CSP03): sc-56070
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
caspase-8 Double Nickase Plasmid (h) | sc-400147-NIC | 20 µg | $410.00 | |||
caspase-8 Double Nickase Plasmid (h2) | sc-400147-NIC-2 | 20 µg | $410.00 |
CASP8 encodes caspase-8, an initiator caspase that couples death receptor signaling to apoptotic execution through proteolytic activation of downstream effector caspases. It is a core component of extrinsic apoptosis mediated by TNFRSF receptors such as FAS and TNFR1, and it also interfaces with innate immune pathways that govern inflammasome crosstalk and cytokine processing. Caspase-8 activity influences the balance between apoptosis, necroptosis, and inflammatory cell death by modulating RIPK1/RIPK3 signaling and cFLIP-dependent complex assembly. Dysregulation of CASP8 has been associated with altered immune homeostasis, defective lymphocyte apoptosis, and tumor cell survival mechanisms that affect sensitivity to death receptor ligands.
caspase-8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CASP8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CASP8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CASP8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CASP8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.