Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CARD 11 Double Nickase Plasmid (h): sc-402770-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CARD 11 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CARD 11 Double Nickase Plasmid (h) and CARD 11 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CARD11. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CARD 11 Antibody (A-4): sc-166910
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CARD 11 Double Nickase Plasmid (h) | sc-402770-NIC | 20 µg | $410.00 |
Human CARD11 (CARD 11/CARMA1) encodes a scaffold protein that links antigen receptor signaling to downstream NF-κB, JNK, and mTOR pathway activation, integrating signals required for lymphocyte activation, proliferation, and cytokine production. Upon stimulation, CARD 11 participates in assembly of the CBM complex (CARD 11–BCL10–MALT1) to promote IKK activation and transcriptional programs controlling immune responses. Dysregulated CARD11 signaling is associated with altered immune homeostasis, including immunodeficiency and lymphoproliferative phenotypes, and recurrent pathway perturbations are observed in certain B- and T-cell malignancies. As a central node in receptor-proximal signaling, CARD 11 is widely studied for mechanisms of signal transduction, transcriptional regulation, and immune cell fate decisions.
CARD 11 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CARD11 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CARD11. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CARD11 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CARD11-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.