CARD 10 Antibody (B-4): sc-271849
- CARD 10 Antibody (B-4) is a mouse monoclonal IgG3 κ CARD 10 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 857-877 near the C-terminus of CARD 10 of human origin
- recommended for detection of CARD 10 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including porcine
- m-IgG3 BP-HRP is the preferred secondary detection reagent for CARD 10 Antibody (B-4) for WB applications. This reagent is now offered in a bundle with CARD 10 Antibody (B-4) (see ordering information below).
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CARD 10 Antibody (B-4) is a mouse monoclonal IgG3 kappa light chain antibody that detects CARD 10 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CARD 10 antibody (B-4) is available as non-conjugated format. CARD 10 protein, also known as CARD-containing MAGUK protein 3 or Carma 3, plays a crucial role in apoptosis signaling by facilitating multiprotein complex assembly at plasma membrane, which is essential for cell death and survival regulation. CARD 10 belongs to membrane-associated guanylate kinase (MAGUK) family, serving as molecular scaffolds that localize signaling proteins, thereby influencing various cellular processes. CARD domain structure, consisting of 6 or 7 antiparallel alpha helices, enables specific protein-protein interactions necessary for activating downstream signaling pathways, including NFκB pathway through BCL-10 interaction. CARD 10 is expressed in various adult and fetal tissues, including heart, kidney, and liver, as well as multiple cancer cell lines, highlighting its importance in both normal physiology and disease states.
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CARD 10 Antibody (B-4) References:
- Cell biology. Reeling CASK into the nucleus. | Bredt, DS. 2000. Nature. 404: 241-2. PMID: 10749196
- Nuclear translocation and transcription regulation by the membrane-associated guanylate kinase CASK/LIN-2. | Hsueh, YP., et al. 2000. Nature. 404: 298-302. PMID: 10749215
- Card10 is a novel caspase recruitment domain/membrane-associated guanylate kinase family member that interacts with BCL10 and activates NF-kappa B. | Wang, L., et al. 2001. J Biol Chem. 276: 21405-9. PMID: 11259443
- Carma1, a CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappaB activation. | Gaide, O., et al. 2001. FEBS Lett. 496: 121-7. PMID: 11356195
- Physical and functional interaction of CARMA1 and CARMA3 with Ikappa kinase gamma-NFkappaB essential modulator. | Stilo, R., et al. 2004. J Biol Chem. 279: 34323-31. PMID: 15184390
- Camguk, Lin-2, and CASK: novel membrane-associated guanylate kinase homologs that also contain CaM kinase domains. | Dimitratos, SD., et al. 1997. Mech Dev. 63: 127-30. PMID: 9178262
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CARD 10 Antibody (B-4) | sc-271849 | 200 µg/ml | $316.00 | |||
CARD 10 Antibody (B-4): m-IgG3 BP-HRP Bundle | sc-550563 | 200 µg Ab; 40 µg BP | $354.00 | |||
CARD 10 (B-4) Neutralizing Peptide | sc-271849 P | 100 µg/0.5 ml | $68.00 |