CapZ- alpha 3 Antibody (MB120) recommended for detection of CapZ-α3 of mouse, rat and human origin by WB and IP

CapZ-α3 Antibody (MB120): sc-130480

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CapZ-α3 Antibody (MB120) is rated 5.0 out of 5 by 1.
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Datasheets

    • Anti-CapZ-α3 Antibody (MB120) is a mouse monoclonal IgG2a (kappa light chain) CapZ-α3 antibody provided at 50 µg/0.5 ml
    • raised against recombinant CapZ-α3 of human origin
    • recommended for detection of CapZ-α3 of mouse, rat and human origin by WB and IP
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

I am using CapZ-α3 (MB120): sc-130480 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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