Molecular structure of CAPS, CAS Number: 1135-40-6
CAPS (CAS 1135-40-6)
Alternate Names:
3-(Cyclohexylamino)-1-propanesulfonic acid
Application:
CAPS is used to formulate CAPS buffer, a zwitterionic buffer useful in the range of pH 7.9-11.1
CAS Number:
1135-40-6
Purity:
≥98%
Molecular Weight:
221.31
Molecular Formula:
C9H19NO3S
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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Description
Technical Information
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SDS & Certificate of Analysis
CAPS (N-cyclohexyl-3-aminopropanesulfonic acid) buffer salt is used to formulate CAPS buffer, a zwitterionic buffer that is useful in the range of pH 7.9-11.1. CAPS buffer is widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electrotransfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8.5. CAPS is not one of the original Good′s buffers, although it has a similar structure to the other propanesulfonic acids and was selected as a highly water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins, minimal salt effects. Also available: CAPSO (sc-239475) CAPSO sodium salt (sc-285280)
CAPS (CAS 1135-40-6) References
- Direct monitoring of the expression of the green fluorescent protein-extracellular signal-regulated kinase 2 fusion protein in transfected cells using capillary electrophoresis with laser-induced fluorescence detection. | Yoon, S., et al. 2002. J Chromatogr A. 976: 87-93. PMID: 12462599
- Simultaneous determination of 5-hydroxyindoles and catechols by high-performance liquid chromatography with fluorescence detection following derivatization with benzylamine and 1,2-diphenylethylenediamine. | Fujino, K., et al. 2003. J Chromatogr A. 1012: 169-77. PMID: 14521313
- Analysis of tryptophan and tyrosine in cerebrospinal fluid by capillary electrophoresis and 'ball lens' UV-pulsed laser-induced fluorescence detection. | Bayle, C., et al. 2003. J Chromatogr A. 1013: 123-30. PMID: 14604114
- The role of CAPS buffer in expanding the crystallization space of the nucleotide-binding domain of the ABC transporter haemolysin B from Escherichia coli. | Zaitseva, J., et al. 2004. Acta Crystallogr D Biol Crystallogr. 60: 1076-84. PMID: 15159567
- Optimised two-dimensional electrophoresis procedures for the protein characterisation of structural tissues. | Hopkinson, A., et al. 2005. Proteomics. 5: 1967-79. PMID: 15816006
- On-line enhancement and separation of nanoparticles using capillary electrophoresis. | Lin, KH., et al. 2007. J Chromatogr A. 1161: 314-21. PMID: 17559859
- A high-efficiency capillary electrophoresis-based method for characterizing the sizes of Au nanoparticles. | Liu, FK. 2007. J Chromatogr A. 1167: 231-5. PMID: 17850805
- Stacking enhanced determination of steroids by CE. | Bykova, L. and Holland, LA. 2008. Electrophoresis. 29: 3794-800. PMID: 18850649
- Evaluating binuclear copper(II) complexes for glycoside hydrolysis. | Striegler, S., et al. 2010. Inorg Chem. 49: 2639-48. PMID: 20166683
- Effect of zwitterionic buffers on measurement of small masses of protein with bicinchoninic acid. | Kaushal, V. and Barnes, LD. 1986. Anal Biochem. 157: 291-4. PMID: 3777432
- The effect of the dimeric and multimeric forms of fibronectin on the adhesion and growth of primary glomerular cells. | Oberley, TD., et al. 1983. Exp Cell Res. 145: 265-76. PMID: 6861897
- Frog striated muscle is permeable to hydroxide and buffer anions. | Venosa, RA., et al. 1994. J Membr Biol. 139: 57-74. PMID: 8071988
- Contribution of capillary zone electrophoresis to the analysis of cecal mucins. | Gómez Treviño, M., et al. 1996. J Capillary Electrophor. 3: 287-94. PMID: 9384722
- Sensitive determination of arsenite by capillary zone electrophoresis with direct photometric detection. | Vanifatova, NG., et al. 1997. J Capillary Electrophor. 4: 91-6. PMID: 9624575
- [Determination of sennoside A and sennoside B in formulation using capillary electrophoresis]. | Satoh, K., et al. 1999. Yakugaku Zasshi. 119: 88-92. PMID: 9922712
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CAPS, 25 g | sc-216090B | 25 g | $36.00 | |||
CAPS, 100 g | sc-216090 | 100 g | $80.00 | |||
CAPS, 250 g | sc-216090C | 250 g | $132.00 | |||
CAPS, 1 kg | sc-216090A | 1 kg | $438.00 | |||
CAPS, 2.5 kg | sc-216090D | 2.5 kg | $989.00 | |||
CAPS, 5 kg | sc-216090E | 5 kg | $1892.00 |