Caprin1 Double Nickase Plasmid (m): sc-424757-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Caprin1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Caprin1 Double Nickase Plasmid (m) and Caprin1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Caprin1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Caprin1 Antibody (F-3): sc-518251
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Caprin1 Double Nickase Plasmid (m) | sc-424757-NIC | 20 µg | $410.00 |
Caprin1 (cell cycle-associated protein 1) is a conserved RNA-binding protein that contributes to post-transcriptional gene regulation by associating with ribonucleoprotein complexes and stress granules. In mouse cells, Caprin1 supports mRNA stability and translation control, linking it to proliferative signaling and adaptive responses to cellular stress. It has been implicated in neuronal and synaptic function, immune-related signaling, and regulation of cell cycle–associated transcripts, aligning it with pathways that coordinate growth, differentiation, and stress adaptation. Dysregulated Caprin1 activity has been associated in the literature with neurodevelopmental phenotypes and altered cell survival programs, making it relevant for mechanistic studies of RNA metabolism and stress-responsive gene expression.
Caprin1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Caprin1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Caprin1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Caprin1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Caprin1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.