CAP1 Antibody (E-4): sc-376191

CAP1 Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects CAP1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CAP1 Antibody (E-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cyclase-associated proteins (CAPs) are a family of evolutionarily conserved proteins that play crucial roles in signal transduction and the regulation of the actin cytoskeleton. CAP1, along with CAP2, is localized to the cell membrane and features a C-CAP/cofactor C-like domain, which is essential for function. CAP1 plays a key role in regulating actin filaments, which is vital for processes such as cell polarity establishment and mRNA localization. These functions are critical for maintaining cellular architecture and facilitating proper cellular responses to environmental cues. While CAP1 is expressed throughout various tissues, CAP2 shows predominant expression in skin, brain, heart, and skeletal muscle, with CAP2 overexpression linked to hepatocellular carcinoma, indicating potential involvement in cancer development.

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CAP1 Antibody (E-4) References:

1. Human CAP1 is a key factor in the recycling of cofilin and actin for rapid actin turnover.  |  Moriyama, K. and Yahara, I. 2002. J Cell Sci. 115: 1591-601. PMID: 11950878
2. Identification of a human cDNA encoding a protein that is structurally and functionally related to the yeast adenylyl cyclase-associated CAP proteins.  |  Matviw, H., et al. 1992. Mol Cell Biol. 12: 5033-40. PMID: 1406678
3. Cyclase-associated protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells.  |  Bertling, E., et al. 2004. Mol Biol Cell. 15: 2324-34. PMID: 15004221
4. Crystal structure of the actin binding domain of the cyclase-associated protein.  |  Dodatko, T., et al. 2004. Biochemistry. 43: 10628-41. PMID: 15311924
5. Overexpression of cyclase-associated protein 2 in multistage hepatocarcinogenesis.  |  Shibata, R., et al. 2006. Clin Cancer Res. 12: 5363-8. PMID: 17000669
6. CAP2, cyclase-associated protein 2, is a dual compartment protein.  |  Peche, V., et al. 2007. Cell Mol Life Sci. 64: 2702-15. PMID: 17805484
7. Adenylyl cyclase-associated protein-1/CAP1 as a biological target substrate of gelatinase B/MMP-9.  |  Cauwe, B., et al. 2008. Exp Cell Res. 314: 2739-49. PMID: 18671965
8. Downregulated expression of the cyclase-associated protein 1 (CAP1) reduces migration in esophageal squamous cell carcinoma.  |  Li, M., et al. 2013. Jpn J Clin Oncol. 43: 856-64. PMID: 23904342
9. Upregulated expression of CAP1 is associated with tumor migration and metastasis in hepatocellular carcinoma.  |  Liu, Y., et al. 2014. Pathol Res Pract. 210: 169-75. PMID: 24359721
10. Phosphorylation of CAP1 regulates lung cancer proliferation, migration, and invasion.  |  Zeng, J., et al. 2022. J Cancer Res Clin Oncol. 148: 137-153. PMID: 34636991
11. Comparison of human CAP and CAP2, homologs of the yeast adenylyl cyclase-associated proteins.  |  Yu, G., et al. 1994. J Cell Sci. 107 (Pt 6): 1671-8. PMID: 7962207
12. Mammalian CAP interacts with CAP, CAP2, and actin.  |  Hubberstey, A., et al. 1996. J Cell Biochem. 61: 459-66. PMID: 8761950

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CAP1 Antibody (E-4) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CAP1 Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CAP1 Antibody (E-4) Specifications

Alternate Product Name: CAP1

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 14-74

Epitope Target: CAP1

Epitope Species: human

Product Reactivity/Detection: CAP1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CAP1 Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin