Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CaMKII gamma Double Nickase Plasmid (h): sc-400681-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CaMKII gamma Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CaMKII gamma Double Nickase Plasmid (h) and CaMKII gamma Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CAMK2G. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CaMKII gamma Antibody (8G10C1): sc-517278
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CaMKII gamma Double Nickase Plasmid (h) | sc-400681-NIC | 20 µg | $410.00 | |||
CaMKII gamma Double Nickase Plasmid (h2) | sc-400681-NIC-2 | 20 µg | $410.00 |
Human CAMK2G encodes CaMKII gamma, a serine/threonine kinase activated by Ca2+/calmodulin that translates calcium transients into phosphorylation programs controlling synaptic plasticity, excitation–transcription coupling, and cytoskeletal remodeling. CaMKII gamma participates in CaMK signaling and downstream MAPK/ERK and CREB-linked transcriptional pathways, influencing cell motility, vesicle trafficking, and activity-dependent gene expression. Altered CAMK2G activity or expression has been associated with dysregulated calcium signaling implicated in neurological phenotypes and excitability-related processes, and it is also studied in contexts of aberrant signaling in proliferative and stress-response pathways.
CaMKII gamma Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CAMK2G locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CAMK2G. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CAMK2G function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CAMK2G-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.