Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CaMKII delta Double Nickase Plasmid (h): sc-400811-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CaMKII delta Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CaMKII delta Double Nickase Plasmid (h) and CaMKII delta Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CAMK2D. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CaMKII delta Antibody (L-04): sc-100362
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CaMKII delta Double Nickase Plasmid (h) | sc-400811-NIC | 20 µg | $410.00 | |||
CaMKII delta Double Nickase Plasmid (h2) | sc-400811-NIC-2 | 20 µg | $410.00 |
CAMK2D encodes CaMKII delta, a Ca2+/calmodulin-dependent serine/threonine kinase that integrates intracellular calcium signals into phosphorylation programs controlling excitation–contraction coupling, cytoskeletal organization, and gene transcription. CaMKII delta participates in Ca2+-dependent signaling cascades downstream of ion channels and G protein–coupled receptors, shaping pathways such as MAPK signaling and transcriptional responses mediated by CREB and related regulators. In human tissues, CAMK2D activity is frequently studied in the context of cardiac and smooth muscle physiology, where altered calcium handling and kinase signaling are linked to maladaptive remodeling phenotypes. Dysregulated CaMKII delta signaling has also been investigated for its broader roles in stress responses, cell survival, and inflammatory signaling networks relevant to cardiometabolic and neurovascular research.
CaMKII delta Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CAMK2D locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CAMK2D. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CAMK2D function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CAMK2D-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.