CaM Kinase II (290-309), Calmodulin Antagonist (CAS 115044-69-4)
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CaM Kinase II (290-309), Calmodulin Antagonist is a potent, synthetic peptide inhibitor of CaMKII (Ca2+/calmodulin-dependent protein kinase II), which was derived from the cDNA sequence of the rat brain 50-kDa alpha subunit. The use of this inhibitory peptide elucidated the calmodulin binding and inhibitory domains, as well as the initial autophosphorylation site (Thr286) of CaMKII with an IC50 of 52 nM. This peptide product binds CaM (calmodulin) tightly and inhibits additional calmodulin-requiring enzymes, such as PDE (phosphodiesterase) and PP2B (calcineurin). Investigators used this product in STC-1 cells to study the regulation of L-type calcium channels and found the inhibitory peptide to block whole cell calcium currents. Additional research in T84 cells showed that this peptide reduced chloride current by approximately 70% without effect on the channels in parotid acinar cells.
CaM Kinase II (290-309), Calmodulin Antagonist (CAS 115044-69-4) References
- Localization, purification, and characterization of the rabbit sarcoplasmic reticulum associated calmodulin-dependent protein kinase. | Pelosi, M. and Donella-Deana, A. 2000. Biochemistry (Mosc). 65: 259-68. PMID: 10713557
- Characteristics of the inhibitory effect of calmodulin on specific [125i]omega-conotoxin GVIA binding to crude membranes from chick brain. | Ichida, S., et al. 2000. Neurochem Res. 25: 1629-35. PMID: 11152392
- Gating of connexin 43 gap junctions by a cytoplasmic loop calmodulin binding domain. | Xu, Q., et al. 2012. Am J Physiol Cell Physiol. 302: C1548-56. PMID: 22422398
- Regulatory domain of calcium/calmodulin-dependent protein kinase II. Mechanism of inhibition and regulation by phosphorylation. | Colbran, RJ., et al. 1989. J Biol Chem. 264: 4800-4. PMID: 2538462
- Calcium/calmodulin-dependent protein kinase II. Characterization of distinct calmodulin binding and inhibitory domains. | Payne, ME., et al. 1988. J Biol Chem. 263: 7190-5. PMID: 2835367
- Mutational analysis of Ca(2+)-independent autophosphorylation of calcium/calmodulin-dependent protein kinase II. | Mukherji, S. and Soderling, TR. 1995. J Biol Chem. 270: 14062-7. PMID: 7775466
- A 60 kDa polypeptide of skeletal-muscle sarcoplasmic reticulum is a calmodulin-dependent protein kinase that associates with and phosphorylates several membrane proteins. | Leddy, JJ., et al. 1993. Biochem J. 295 (Pt 3): 849-56. PMID: 8240301
- Differences in regulation of Ca(2+)-activated Cl- channels in colonic and parotid secretory cells. | Arreola, J., et al. 1998. Am J Physiol. 274: C161-6. PMID: 9458724
- Activation of calcium channels by cAMP in STC-1 cells is dependent upon Ca2+ calmodulin-dependent protein kinase II. | Basavappa, S., et al. 1999. Biochem Biophys Res Commun. 254: 699-702. PMID: 9920804
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CaM Kinase II (290-309), Calmodulin Antagonist, 500 µg | sc-201158 | 500 µg | $145.00 |