Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Calretinin Double Nickase Plasmid (h): sc-401389-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Calretinin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Calretinin Double Nickase Plasmid (h) and Calretinin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CALB2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Calretinin Antibody (H-5): sc-365956
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calretinin Double Nickase Plasmid (h) | sc-401389-NIC | 20 µg | $410.00 | |||
Calretinin Double Nickase Plasmid (h2) | sc-401389-NIC-2 | 20 µg | $410.00 |
Human CALB2 encodes calretinin, an EF-hand calcium-binding protein that buffers intracellular Ca2+ and shapes calcium-dependent excitability, synaptic transmission, and activity-dependent gene regulation. Calretinin is enriched in specific interneuron populations and also expressed in select peripheral tissues, where it contributes to cytoskeletal dynamics and vesicular trafficking through Ca2+-regulated signaling. CALB2 is commonly used as a molecular marker of neuronal subtype identity and mesothelial differentiation, linking its expression to studies of neural circuit organization and epithelial lineage programs. Dysregulated CALB2 expression and altered calcium homeostasis have been associated with neurodevelopmental and neurodegenerative contexts and with tumor biology in certain tissue types, supporting its value for mechanistic research.
Calretinin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CALB2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CALB2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CALB2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CALB2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.