Calpain reg Double Nickase Plasmid (h): sc-403478-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Calpain reg Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Calpain reg Double Nickase Plasmid (h) and Calpain reg Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CAPNS1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Calpain reg Antibody (P-1): sc-32325
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calpain reg Double Nickase Plasmid (h) | sc-403478-NIC | 20 µg | $410.00 | |||
Calpain reg Double Nickase Plasmid (h2) | sc-403478-NIC-2 | 20 µg | $410.00 |
CAPNS1 encodes the regulatory small subunit required for stability and activity of the ubiquitous calpain-1 and calpain-2 cysteine protease complexes. Calpain signaling mediates limited proteolysis of cytoskeletal and signaling proteins, shaping focal adhesion turnover, membrane dynamics, and calcium-dependent remodeling during cell migration, cell-cycle progression, and apoptosis. Through cleavage of substrates involved in NF-κB, MAPK, and integrin-associated pathways, CAPNS1 can influence inflammatory signaling and stress responses. Dysregulated calpain activity has been linked to cancer cell invasiveness, neurodegeneration, and cardiovascular and metabolic pathophysiology, making CAPNS1 a useful node for mechanistic studies of proteostasis and calcium-driven signaling networks.
Calpain reg Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CAPNS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CAPNS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CAPNS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CAPNS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.