Calpain 6 Antibody (1395CT446.22.5): sc-517318

Calpain 6 Antibody (1395CT446.22.5) is a mouse monoclonal IgG1 antibody that detects Calpain 6 protein of human origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). Anti-Calpain 6 antibody (1395CT446.22.5) is available as a non-conjugated format. Calpain 6, also known as CAPN6, is a member of the calpain family of calcium-activated thiol proteases, which play crucial roles in intracellular protein processing and signal transduction. Notably, CAPN6 is primarily expressed in the placenta, where CAPN6 is believed to be involved in embryogenesis, highlighting CAPN6′s importance in developmental processes. Structurally, CAPN6 is unique among its family members due to the presence of a T-domain that is homologous to the Caenorhabditis elegans protein Tra-3, which may suggest a specialized function in cellular processes. Unlike other calpains, CAPN6 lacks the cysteine and histidine residues necessary for protease activity, indicating that CAPN6 may have alternative roles in cellular dynamics, such as actin organization and microtubule stability. Overexpression of CAPN6 has been linked to cytokinesis failure and the formation of microtubule bundles, resulting in cells with multiple nuclei, which underscores CAPN6′s potential impact on cell division and organization.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Calpain 6 Antibody (1395CT446.22.5) References:

1. Slug is a novel downstream target of MyoD. Temporal profiling in muscle regeneration.  |  Zhao, P., et al. 2002. J Biol Chem. 277: 30091-101. PMID: 12023284
2. Down-regulation of calpain 9 is linked to hypertensive heart and kidney disease.  |  Markmann, A., et al. 2005. Cell Physiol Biochem. 15: 109-16. PMID: 15665521
3. Spectrin and calpain: a 'target' and a 'sniper' in the pathology of neuronal cells.  |  Czogalla, A. and Sikorski, AF. 2005. Cell Mol Life Sci. 62: 1913-24. PMID: 15990959
4. Calpain is activated in degenerating photoreceptors in the rd1 mouse.  |  Paquet-Durand, F., et al. 2006. J Neurochem. 96: 802-14. PMID: 16405498
5. Thiol proteases: inhibitors and potential therapeutic targets.  |  Leung-Toung, R., et al. 2006. Curr Med Chem. 13: 547-81. PMID: 16515521
6. Calpain 6 is involved in microtubule stabilization and cytoskeletal organization.  |  Tonami, K., et al. 2007. Mol Cell Biol. 27: 2548-61. PMID: 17210638
7. Increased expression of calpain 6 in uterine sarcomas and carcinosarcomas: an immunohistochemical analysis.  |  Lee, SJ., et al. 2007. Int J Gynecol Cancer. 17: 248-53. PMID: 17291261
8. The calpains: modular designs and functional diversity.  |  Croall, DE. and Ersfeld, K. 2007. Genome Biol. 8: 218. PMID: 17608959
9. Emerging functions of the calpain superfamily of cysteine proteases in neuroendocrine secretory pathways.  |  Evans, JS. and Turner, MD. 2007. J Neurochem. 103: 849-59. PMID: 17666040
10. Deficiency of calpain-6 inhibits primary ciliogenesis.  |  Kim, BH., et al. 2019. BMB Rep. 52: 619-624. PMID: 31619317
11. A calpain-6/YAP axis in sarcoma stem cells that drives the outgrowth of tumors and metastases.  |  Tchicaya-Bouanga, J., et al. 2022. Cell Death Dis. 13: 819. PMID: 36153320
12. Calpain and Cardiometabolic Diseases.  |  Miyazaki, T. 2023. Int J Mol Sci. 24: PMID: 38069105