CALCOCO2 Double Nickase Plasmid (h): sc-416970-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CALCOCO2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CALCOCO2 Double Nickase Plasmid (h) and CALCOCO2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CALCOCO2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CALCOCO2 Antibody (F-6): sc-376540
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CALCOCO2 Double Nickase Plasmid (h) | sc-416970-NIC | 20 µg | $410.00 | |||
CALCOCO2 Double Nickase Plasmid (h2) | sc-416970-NIC-2 | 20 µg | $410.00 |
CALCOCO2 (also known as NDP52) is a selective autophagy receptor that links ubiquitinated cargo to the autophagosome through LC3/GABARAP family interactions, coordinating xenophagy, mitophagy, and turnover of protein aggregates. It acts at the interface of innate immune signaling and membrane trafficking, functioning downstream of ubiquitin-dependent recognition pathways and contributing to pathogen restriction and inflammatory control. CALCOCO2 participates in vesicle dynamics and stress-response networks that shape cellular homeostasis, including crosstalk with NF-κB–associated signaling and cytoskeletal organization. Dysregulation of CALCOCO2-mediated autophagy and immune surveillance has been studied in contexts such as infection biology, inflammatory phenotypes, and cancer-related cell survival programs.
CALCOCO2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CALCOCO2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CALCOCO2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CALCOCO2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CALCOCO2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.