Cal-520™, AM
See product citations (2)
QUICK LINKS
Cal-520™, AM provides a robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization. It is a new fluorogenic calcium-sensitive dye with a significantly improved signal to noise ratio and intracellular retention compared to the existing green calcium indicators (such as Fluo-3 AM and Fluo-4 AM). Cells expressing a GPCR or calcium channel of interest that signals through calcium can be preloaded with Cal-520™, AM which can cross the cell membrane. The characteristics of its long wavelength, high sensitivity, and >100 times fluorescence enhancement, make Cal-520™, AM an ideal indicator for the measurement of cellular calcium. The high S/N ratio and better intracellular retention make the Cal-520™, AM calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists.
Cal-520™, AM References
- Modelling cardiac calcium sparks in a three-dimensional reconstruction of a calcium release unit. | Hake, J., et al. 2012. J Physiol. 590: 4403-22. PMID: 22495592
- Regulation of calcium clock-mediated pacemaking by inositol-1,4,5-trisphosphate receptors in mouse sinoatrial nodal cells. | Kapoor, N., et al. 2015. J Physiol. 593: 2649-63. PMID: 25903031
- A comparison of fluorescent Ca²⁺ indicators for imaging local Ca²⁺ signals in cultured cells. | Lock, JT., et al. 2015. Cell Calcium. 58: 638-48. PMID: 26572560
- Cortisol-induced immune suppression by a blockade of lymphocyte egress in traumatic brain injury. | Dong, T., et al. 2016. J Neuroinflammation. 13: 197. PMID: 27561600
- Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice. | Li, J., et al. 2017. Biomed Opt Express. 8: 2599-2610. PMID: 28663893
- Calcium Transient Assays for Compound Screening with Human iPSC-derived Cardiomyocytes: Evaluating New Tools. | Daily, NJ., et al. 2017. J Evol Stem Cell Res. 1: 1-11. PMID: 28966998
- Porphyromonas gingivalis lipopolysaccharide rapidly activates trigeminal sensory neurons and may contribute to pulpal pain. | Kaewpitak, A., et al. 2020. Int Endod J. 53: 846-858. PMID: 32058593
- Transient Cell Membrane Disruptions induce Calcium Waves in Corneal Keratocytes. | Chen, Z., et al. 2020. Sci Rep. 10: 2840. PMID: 32071321
- Implantable photonic neural probes for light-sheet fluorescence brain imaging. | Sacher, WD., et al. 2021. Neurophotonics. 8: 025003. PMID: 33898636
- Cholinergic calcium responses in cultured antennal lobe neurons of the migratory locust. | Bergmann, GA. and Bicker, G. 2021. Sci Rep. 11: 10018. PMID: 33976252
- A protocol for detecting elemental calcium signals (Ca2+ puffs) in mammalian cells using total internal reflection fluorescence microscopy. | Arige, V., et al. 2021. STAR Protoc. 2: 100618. PMID: 34195673
- Fluorescence-coupled micropipette aspiration assay to examine calcium mobilization caused by red blood cell mechanosensing. | Wang, H., et al. 2022. Eur Biophys J. 51: 135-146. PMID: 35286429
- Tubulin-mediated anatomical and functional changes caused by Ca2+ in human erythrocytes. | Balach, MM., et al. 2023. J Physiol Biochem.. PMID: 36773113
- Mechanisms underlying TNF-alpha effects on agonist-mediated calcium homeostasis in human airway smooth muscle cells. | Amrani, Y., et al. 1997. Am J Physiol. 273: L1020-8. PMID: 9374730
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cal-520™, AM, 1 mg | sc-477280 | 1 mg | $377.00 |