Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CAF-1 p150 Double Nickase Plasmid (h): sc-402472-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CAF-1 p150 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CAF-1 p150 Double Nickase Plasmid (h) and CAF-1 p150 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CHAF1A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CAF-1 p150 Antibody (D-1): sc-133105
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CAF-1 p150 Double Nickase Plasmid (h) | sc-402472-NIC | 20 µg | $410.00 | |||
CAF-1 p150 Double Nickase Plasmid (h2) | sc-402472-NIC-2 | 20 µg | $410.00 |
CHAF1A encodes the p150 subunit of chromatin assembly factor 1 (CAF-1), a histone chaperone that deposits H3–H4 onto newly synthesized DNA during S phase and following DNA damage. CAF-1 p150 coordinates replication-coupled nucleosome assembly, epigenetic inheritance, and restoration of chromatin structure after repair, linking chromatin dynamics to genome stability. Through interactions with PCNA and replication/repair machinery, CHAF1A influences DNA replication, homologous recombination, and checkpoint signaling pathways. Dysregulation of CAF-1 activity is associated with replication stress, altered transcriptional programs, and genomic instability phenotypes observed in cancer biology and other chromatin-related disease models.
CAF-1 p150 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CHAF1A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CHAF1A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CHAF1A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CHAF1A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.