cadherin-7 Double Nickase Plasmid (h): sc-401847-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cadherin-7 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- cadherin-7 Double Nickase Plasmid (h) and cadherin-7 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDH7. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cadherin-7 Double Nickase Plasmid (h) | sc-401847-NIC | 20 µg | $410.00 | |||
cadherin-7 Double Nickase Plasmid (h2) | sc-401847-NIC-2 | 20 µg | $410.00 |
CDH7 encodes cadherin-7, a calcium-dependent cell–cell adhesion molecule that supports selective cell recognition and maintenance of tissue architecture, particularly in neural and epithelial contexts. Through homophilic binding and linkage to catenins, cadherin-7 helps coordinate adherens junction organization, cytoskeletal dynamics, and contact-dependent signaling programs that influence cell migration and differentiation. Altered cadherin-mediated adhesion is frequently associated with changes in morphogenesis, epithelial–mesenchymal plasticity, and invasive phenotypes, making CDH7 a relevant node in studies of tumor cell dissemination and neurodevelopmental processes. As part of the broader cadherin network, CDH7 perturbation can be used to interrogate how adhesion cues integrate with pathways controlling polarity, junction stability, and transcriptional state.
cadherin-7 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDH7 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDH7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDH7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDH7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.