Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cacna2d1 Double Nickase Plasmid (h): sc-402833-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cacna2d1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cacna2d1 Double Nickase Plasmid (h) and Cacna2d1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CACNA2D1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cacna2d1 Antibody (E-10): sc-271697
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cacna2d1 Double Nickase Plasmid (h) | sc-402833-NIC | 20 µg | $410.00 | |||
Cacna2d1 Double Nickase Plasmid (h2) | sc-402833-NIC-2 | 20 µg | $410.00 |
CACNA2D1 encodes the α2δ-1 auxiliary subunit of voltage-gated calcium channels, a key regulator of channel trafficking, membrane stability, and gating that shapes calcium influx during excitability and stimulus–secretion coupling. By modulating CaV channel function, Cacna2d1 influences calcium-dependent signaling cascades that impact neurotransmitter release, muscle contraction, and activity-dependent gene expression. Altered CACNA2D1 expression or channel complex regulation has been linked to dysregulated electrophysiology and remodeling processes relevant to neurological and cardiovascular research contexts. As a surface-exposed component of the channel complex, Cacna2d1 is also useful for dissecting how extracellular interactions and post-translational modifications tune calcium channel availability.
Cacna2d1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CACNA2D1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CACNA2D1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CACNA2D1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CACNA2D1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.