C9orf152 Double Nickase Plasmid (h): sc-415979-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C9orf152 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- C9orf152 Double Nickase Plasmid (h) and C9orf152 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting C9orf152. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C9orf152 Double Nickase Plasmid (h) | sc-415979-NIC | 20 µg | $410.00 | |||
C9orf152 Double Nickase Plasmid (h2) | sc-415979-NIC-2 | 20 µg | $410.00 |
C9orf152 is a poorly characterized human protein-coding gene with limited functional annotation, and current evidence suggests roles in fundamental cellular regulation rather than a single pathway-specific function. Expression profiling and emerging proteogenomic datasets can help position C9orf152 within broader processes such as transcriptional control, RNA metabolism, and cellular stress responses. Because uncharacterized open reading frame genes often show context-dependent essentiality, perturbation of C9orf152 is useful for mapping genetic dependencies across cell types. Such studies support investigation of potential associations with complex disease phenotypes through functional genomics and variant interpretation, without presuming a defined pathogenic mechanism.
C9orf152 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the C9orf152 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within C9orf152. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt C9orf152 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of C9orf152-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.