Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
C1QBP Double Nickase Plasmid (h): sc-400911-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C1QBP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- C1QBP Double Nickase Plasmid (h) and C1QBP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting C1QBP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: C1QBP Antibody (H-9): sc-271200
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C1QBP Double Nickase Plasmid (h) | sc-400911-NIC | 20 µg | $410.00 | |||
C1QBP Double Nickase Plasmid (h2) | sc-400911-NIC-2 | 20 µg | $410.00 |
C1QBP (complement component 1q binding protein; also known as p32/gC1qR) is a multifunctional human protein that localizes predominantly to mitochondria and participates in regulation of oxidative phosphorylation, mitochondrial ribosome function, and cellular energy metabolism. It also interacts with components of the complement system and can influence inflammatory signaling and innate immune processes in a context-dependent manner. Through roles in mitochondrial homeostasis and RNA/protein handling, C1QBP contributes to control of apoptosis, stress responses, and proliferation programs. Altered C1QBP expression or localization has been associated with dysregulated metabolism and inflammatory phenotypes reported across multiple disease contexts, supporting its utility as a mechanistic target in immunometabolism and mitochondrial biology research.
C1QBP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the C1QBP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within C1QBP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt C1QBP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of C1QBP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.