C1orf85 Double Nickase Plasmid (h): sc-414006-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C1orf85 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- C1orf85 Double Nickase Plasmid (h) and C1orf85 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GLMP. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C1orf85 Double Nickase Plasmid (h) | sc-414006-NIC | 20 µg | $410.00 | |||
C1orf85 Double Nickase Plasmid (h2) | sc-414006-NIC-2 | 20 µg | $410.00 |
GLMP encodes C1orf85, a lysosomal and endolysosomal membrane-associated glycoprotein implicated in maintaining lysosome integrity and supporting normal trafficking and turnover of macromolecules. Functional studies link GLMP to lysosomal homeostasis, membrane protein stability, and cellular responses to metabolic stress, consistent with roles in autophagy-lysosome pathways and intracellular catabolism. Disruption of GLMP has been associated with lysosomal dysfunction phenotypes and has been investigated in the context of metabolic and hepatic pathology, where impaired lysosomal processing can contribute to cellular injury. As a result, GLMP/C1orf85 is a useful target for dissecting how lysosome-dependent quality control influences inflammation, proteostasis, and organelle crosstalk in human cells.
C1orf85 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GLMP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GLMP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GLMP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GLMP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.