C19orf57 Double Nickase Plasmid (h): sc-413149-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C19orf57 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- C19orf57 Double Nickase Plasmid (h) and C19orf57 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting C19orf57. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C19orf57 Double Nickase Plasmid (h) | sc-413149-NIC | 20 µg | $410.00 | |||
C19orf57 Double Nickase Plasmid (h2) | sc-413149-NIC-2 | 20 µg | $410.00 |
C19orf57 encodes a small human protein with limited functional annotation, reported in several datasets to localize to the nucleus and associate with processes linked to RNA metabolism and cell-cycle–coupled genome maintenance. Transcript and proteomic studies suggest context-dependent regulation across proliferative states, supporting investigation of roles in transcriptional control, RNA processing, and replication-associated stress responses. Altered expression patterns for C19orf57 have been observed in multiple disease-relevant datasets, including cancer-associated profiling, making it a useful locus for mechanistic studies of gene regulation and cellular fitness. Because pathway placement remains incompletely defined, C19orf57 serves as a candidate gene for unbiased loss-of-function screens and network mapping in human cells.
C19orf57 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the C19orf57 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within C19orf57. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt C19orf57 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of C19orf57-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.