C14orf119 Double Nickase Plasmid (h): sc-412417-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C14orf119 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- C14orf119 Double Nickase Plasmid (h) and C14orf119 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting C14orf119. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C14orf119 Double Nickase Plasmid (h) | sc-412417-NIC | 20 µg | $410.00 | |||
C14orf119 Double Nickase Plasmid (h2) | sc-412417-NIC-2 | 20 µg | $410.00 |
C14orf119 (human) encodes a poorly characterized protein with limited functional annotation and sparse pathway mapping in current curated resources. Available transcript and protein evidence suggests it may contribute to basic cellular processes such as gene expression regulation, protein localization, or context-dependent signaling, but its specific molecular partners and mechanistic role remain to be defined. Expression profiling and comparative analyses can be used to explore potential links to cell state transitions, stress responses, or lineage-specific programs. As an under-studied open reading frame, C14orf119 is relevant for functional genomics efforts aimed at connecting genotype to phenotype in disease-associated contexts without presupposing a defined pathway.
C14orf119 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the C14orf119 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within C14orf119. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt C14orf119 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of C14orf119-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.