c-Kit Antibody (104D2): sc-19983

c-Kit Antibody (104D2) is a mouse monoclonal IgG1 kappa light chain antibody designed to target CD117 expressed on the human megakaryocytic cell line MOLM-1, demonstrating strong reactivity in human samples when used in immunofluorescence (IF) and flow cytometry (FCM) applications. The c-Kit protein, also known as CD117, is a transmembrane receptor tyrosine kinase that plays a critical role in various cellular processes such as chemotaxis, proliferation, apoptosis, and adhesion. Structurally, c-Kit consists of an extracellular domain responsible for ligand binding, a single transmembrane helix, and an intracellular tyrosine kinase domain that initiates downstream signaling pathways upon activation by its ligand, stem cell factor (SCF). This structural configuration is essential for its function, as the extracellular domain ensures precise ligand recognition and binding, while the intracellular kinase domain transduces signals that regulate key biological activities including hematopoiesis, mast cell development, melanogenesis, and gametogenesis. The integrity of c-Kit′s structure is crucial for maintaining proper signal transduction. Any alterations or mutations, particularly in the kinase domain, can lead to aberrant signaling that contributes to the development and progression of various cancers, such as gastrointestinal stromal tumors (GISTs) and certain leukemias. c-Kit (104D2) monoclonal antibody is recommended for researchers studying oncology, hematology, and cellular biology.

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c-Kit Antibody (104D2) References:

1. The biology of stem cell factor and its receptor C-kit.  |  Ashman, LK. 1999. Int J Biochem Cell Biol. 31: 1037-51. PMID: 10582338
2. Molecular mechanisms utilized by alternative c-kit gene products in the control of spermatogonial proliferation and sperm-mediated egg activation.  |  Rossi, P., et al. 2003. Andrologia. 35: 71-8. PMID: 12558531
3. A review of soluble c-kit (s-kit) as a novel tumor marker and possible molecular target for the treatment of CNS germinoma.  |  Takeshima, H. and Kuratsu, J. 2003. Surg Neurol. 60: 321-4; discussion 324-5. PMID: 14505850
4. c-kit gene mutations in intracranial germinomas.  |  Sakuma, Y., et al. 2004. Cancer Sci. 95: 716-20. PMID: 15471556
5. c-Kit is essential for alveolar maintenance and protection from emphysema-like disease in mice.  |  Lindsey, JY., et al. 2011. Am J Respir Crit Care Med. 183: 1644-52. PMID: 21471107
6. C-KIT signaling depends on microphthalmia-associated transcription factor for effects on cell proliferation.  |  Phung, B., et al. 2011. PLoS One. 6: e24064. PMID: 21887372
7. Reassessment of c-Kit in Cardiac Cells: A Complex Interplay Between Expression, Fate, and Function.  |  Zhou, B. and Wu, SM. 2018. Circ Res. 123: 9-11. PMID: 29929968
8. The Proto-oncogene c-Kit Inhibits Tumor Growth by Behaving as a Dependence Receptor.  |  Wang, H., et al. 2018. Mol Cell. 72: 413-425.e5. PMID: 30293784
9. Prognostic and predictive significance of KIT protein expression and c-kit gene mutation in canine cutaneous mast cell tumours: A consensus of the Oncology-Pathology Working Group.  |  Thamm, DH., et al. 2019. Vet Comp Oncol. 17: 451-455. PMID: 31264352
10. Interstitial cells of Cajal in Wsh/Wsh c-kit mutant mice.  |  Iino, S., et al. 2020. J Smooth Muscle Res. 56: 58-68. PMID: 33132281

The preferred c-Kit antibody is c-Kit Antibody (E-3): sc-365504.