Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
c-Fos Double Nickase Plasmid (h): sc-400009-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- c-Fos Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- c-Fos Double Nickase Plasmid (h) and c-Fos Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FOS. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: c-Fos Antibody (D-1): sc-8047
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
c-Fos Double Nickase Plasmid (h) | sc-400009-NIC | 20 µg | $410.00 | |||
c-Fos Double Nickase Plasmid (h2) | sc-400009-NIC-2 | 20 µg | $410.00 |
Human FOS encodes c-Fos, an immediate-early transcription factor that dimerizes with JUN family proteins to form AP-1 and rapidly couples extracellular stimuli to gene expression programs. c-Fos is induced downstream of MAPK/ERK, JNK, p38, and calcium-dependent signaling and regulates transcriptional networks controlling proliferation, differentiation, stress responses, apoptosis, and cell motility. Through AP-1–dependent control of cytokines, matrix remodeling enzymes, and cell-cycle regulators, FOS integrates inflammatory and mitogenic cues across many cell types. Dysregulated FOS/AP-1 activity is frequently used as a molecular readout of pathway activation and has been associated with oncogenic signaling, aberrant immune activation, and neuronal plasticity changes in disease-relevant contexts.
c-Fos Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FOS locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FOS. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FOS function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FOS-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.