c-Fos Antibody (6-2H-2F): sc-447

c-Fos Antibody (6-2H-2F) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 139-211 of human c-Fos, a region containing the essential leucine zipper dimerization domain. c-Fos mouse monoclonal antibody (6-2H-2F) recognizes an epitope within this leucine zipper region and demonstrates broad species cross-reactivity, detecting c-Fos, FosB, Fra-1, and Fra-2 proteins in mouse, rat, and human samples through Western blotting (WB) and immunoprecipitation (IP) applications. Anti-c-Fos monoclonal antibody (6-2H-2F) is available in non-conjugated form and multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. The structural organization of c-Fos reveals sophisticated regulation through its leucine zipper motif, which enables formation of specific heterodimers with Jun family proteins such as c-Jun. These heterodimeric complexes constitute the Activator Protein-1 (AP-1) transcription factor, which binds to specific DNA response elements to control gene expression. c-Fos functions as a nuclear phosphoprotein and immediate early gene, responding rapidly and transiently to various cellular signals including growth factors and stress stimuli. Through this dynamic regulation and ability to modulate gene expression, c-Fos serves as a critical coordinator of cellular processes including proliferation, differentiation, and survival. The importance of c-Fos′s structural integrity and proper function becomes particularly evident in pathological conditions where dysregulation contributes to oncogenesis. Through these diverse cellular roles, c-Fos (6-2H-2F) antibody provides researchers with a valuable tool for investigating transcriptional regulation and cellular signaling pathways.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-c-Fos Antibody (6-2H-2F) has 70 citations in a variety of scientific publications.

Primary antibodies like Anti-c-Fos Antibody (6-2H-2F) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

c-Fos Antibody (6-2H-2F) Specifications

Alternate Product Name: c-Fos

Product Clone Name: 6-2H-2F

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 139-211

Epitope Target: c-Fos

Epitope Species: human

Product Reactivity/Detection: c-Fos, Fos B, Fra-1 and Fra-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 70 citations in various scientific publications

Available Conjugates/Forms for c-Fos Antibody (6-2H-2F): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred c-Fos antibody is c-Fos Antibody (D-1): sc-8047.