C/EBP α Antibody (D-5): sc-365318

C/EBP α Antibody (D-5) is a mouse monoclonal IgM antibody that detects C/EBP alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). C/EBP α Antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. C/EBP alpha protein, a member of the CCAAT/enhancer-binding protein family, plays a crucial role in regulating gene expression across various tissues, including liver, adipose tissue, lung, and intestine. C/EBP alpha is involved in the differentiation of several cell types, such as liver cells and adipocytes, and is essential for terminal differentiation of these cells. C/EBP alpha structure features a basic region/leucine zipper motif that facilitates specific binding to DNA, allowing C/EBP alpha to act as a transcription factor regulating genes associated with growth and metabolism. Growth hormone activates C/EBP alpha, increasing C/EBP alpha mRNA and protein levels while enhancing DNA-binding activity, thereby influencing expression of genes like prolactin. C/EBP alpha function in terminally differentiated, growth-arrested cells underscores C/EBP alpha significance in maintaining cellular identity and function, making anti-C/EBP alpha antibody (D-5) an invaluable tool for researchers studying gene regulation and cellular differentiation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

C/EBP α Antibody (D-5) References:

1. CCAAT/enhancer-binding protein alpha is a physiological regulator of prolactin gene expression.  |  Jacob, KK. and Stanley, FM. 1999. Endocrinology. 140: 4542-50. PMID: 10499509
2. Growth hormone induces CCAAT/enhancer binding protein alpha (C/EBPalpha) in cultured rat hepatocytes.  |  Strand, P., et al. 2000. J Hepatol. 32: 618-26. PMID: 10782911
3. Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells.  |  Cao, Z., et al. 1991. Genes Dev. 5: 1538-52. PMID: 1840554
4. Tissue-specific expression, developmental regulation, and genetic mapping of the gene encoding CCAAT/enhancer binding protein.  |  Birkenmeier, EH., et al. 1989. Genes Dev. 3: 1146-56. PMID: 2792758
5. Identification of a rat liver nuclear protein that binds to the enhancer core element of three animal viruses.  |  Johnson, PF., et al. 1987. Genes Dev. 1: 133-46. PMID: 2824279
6. Isolation of a recombinant copy of the gene encoding C/EBP.  |  Landschulz, WH., et al. 1988. Genes Dev. 2: 786-800. PMID: 2850264
7. The DNA binding activity of C/EBP transcription factor is regulated in the G1 phase of the hepatocyte cell cycle.  |  Rana, B., et al. 1995. J Biol Chem. 270: 18123-32. PMID: 7629124
8. Transcription factors C/EBP alpha, C/EBP beta, and CHOP (Gadd153) expressed during the differentiation program of keratinocytes in vitro and in vivo.  |  Maytin, EV. and Habener, JF. 1998. J Invest Dermatol. 110: 238-46. PMID: 9506442
9. Induction of a subgroup of acute phase protein genes in mouse liver by hyperthermia.  |  Yiangou, M., et al. 1998. Biochim Biophys Acta. 1396: 191-206. PMID: 9540835

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-C/EBP α Antibody (D-5) has 64 citations in a variety of scientific publications.

Primary antibodies like Anti-C/EBP α Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

C/EBP α Antibody (D-5) Specifications

Alternate Product Name: C/EBP alpha

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 2-25

Epitope Target: C/EBP α

Epitope Species: human

Product Reactivity/Detection: C/EBP α p42

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 64 citations in various scientific publications

Available Conjugates/Forms for C/EBP α Antibody (D-5): AF488: Alexa Fluor^® 488, P: Blocking Peptide, X: TransCruz Reagent

The preferred C/EBP α antibody is C/EBP α Antibody (G-10): sc-166258.