Bu-1a Antibody (5K98): sc-70447

Bu-1a Antibody (5K98) is a mouse monoclonal IgG1 antibody that detects Bu-1 in avian species through applications such as immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Bu-1 is a genetically polymorphic cell surface antigen that plays a crucial role in the regulation of cell death, particularly during the development of B lymphocytes. This type I transmembrane glycoprotein is essential for maintaining immune homeostasis by ensuring that nonfunctional or self-reactive lymphocytes are effectively removed from the immune repertoire. Bu-1 is expressed not only on B cells but also on a subset of macrophages, highlighting Bu-1′s importance in both adaptive and innate immunity. Additionally, Bu-1 expression on embryonic spleen and bone marrow cells marks these tissues as precursors for B cell development, indicating Bu-1′s role in early immune system formation. Bu-1′s ability to induce a rapid form of cell death akin to apoptosis underscores Bu-1′s significance in controlling cell survival and adhesion during B cell maturation. The two allelic forms of the Bu-1 gene, Bu-1a and Bu-1b, represent the recessive and dominant variants, respectively, further illustrating the complexity of immune regulation mediated by Bu-1. Bu-1a (5K98) monoclonal antibody serves as an invaluable tool for researchers studying B cell development and immune responses in avian species.