Anti-Bu-1a Antibody (5K93) recommended for detection of Bu-1a on bursal cells, thymocytes, spleen cells and peripheral blood cells of avian origin by IP, IF and FCM

Anti-Bu-1a Antibody (5K93): sc-70448

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Anti-Bu-1a Antibody (5K93) is rated 2.0 out of 5 by 1.
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Datasheets

    • Bu-1a Antibody (5K93) is a mouse monoclonal IgG1 provided at 100 µg/ml
    • raised against bursal cells from one day old H.B15 (Bu-1a/b/b) strain avian
    • recommended for detection of Bu-1a on bursal cells, thymocytes, spleen cells and peripheral blood cells of avian origin by IP, IF and FCM; non cross-reactive with cells from CHA and H.B15 strains by immunofluorescence
    • available conjugated to FITC for IF, IHC(P) and FCM
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Bu-1a Antibody (5K93). This work is in progress.
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If I want to use Bu-1a (5K93): sc-70448 for Flow Cytometry, what starting dilution do you recommend I use?

Asked by: Professor Griffin
We recommend a starting dilution of 1µg of antibody per million cells. Here is a link to our recommended protocol: https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-03-24
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