BTNL9 Double Nickase Plasmid (h2): sc-414736-NIC-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BTNL9 Double Nickase Plasmid (h2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BTNL9 Double Nickase Plasmid (h2) and BTNL9 Double Nickase Plasmid (h22) encode distinct paired gRNA designs targeting BTNL9. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BTNL9 Double Nickase Plasmid (h2) | sc-414736-NIC-2 | 20 µg | $410.00 |
Human BTNL9 (butyrophilin-like 9) encodes an immunoglobulin superfamily member implicated in regulating immune cell communication at epithelial and mucosal interfaces, with proposed roles in modulating T cell and innate-like lymphocyte responses through butyrophilin-related co-regulatory mechanisms. BTNL9 expression patterns suggest involvement in immune surveillance, barrier homeostasis, and inflammatory signaling pathways that shape local cytokine milieus and antigen-driven activation states. Genetic and transcriptomic studies have linked altered BTNL9 activity to immune-mediated disorders and cancer-associated immune microenvironment changes, supporting its use as a molecular handle for studying immunoregulation in disease contexts. Gene editing of BTNL9 in human cell models enables functional dissection of BTNL family signaling, assessment of effects on lymphocyte activation and epithelial-immune crosstalk, and validation of BTNL9-associated variants in mechanistic workflows.
BTNL9 Double Nickase Plasmid (h2) consists of a matched pair of plasmids engineered for high-specificity editing of the BTNL9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BTNL9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BTNL9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BTNL9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.