Date published: 2025-9-5
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Btk Antibody (E-9) - Western Blotting - Image 9926
Btk 抗体 (E-9): sc-28387. MEG-01 (A), NAMALWA (B), BJAB (C) 和 U-698-M (D) 全细胞裂解液中 Btk 表达的 Western 印迹分析.
Btk 抗体 (E-9): sc-28387. MEG-01 (A), NAMALWA (B), BJAB (C) 和 U-698-M (D) 全细胞裂解液中 Btk 表达的 Western 印迹分析.

Btk 抗体 (E-9): sc-28387

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说明书
  • Btk抗体(E-9)是小鼠单克隆IgG1 λ Btk抗体, 在16篇文献中引用，规格为200 µg/ml
  • 免疫mouse物种的Btk的氨基酸1-391
  • Btk 抗体 (E-9) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Btk
  • 抗Btk抗体（E-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Btk (E-9): sc-28387的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGλ BP-HRP是Btk Antibody (E-9) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与Btk Antibody (E-9) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Btk抗体（E-9）是一种小鼠单克隆IgG1抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的Btk蛋白。Btk (E-9) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。Btk 也称为布鲁顿酪氨酸激酶，在 B 细胞发育和信号传导中起着至关重要的作用，因此 Btk 对于免疫反应至关重要。Btk基因突变可导致X连锁无丙种球蛋白血症，其特征是抗体生成显著减少，感染风险增加，这凸显了Btk在维持免疫系统完整性方面的重要性。Btk主要位于细胞质中，在B细胞受体激活时被招募到细胞膜上，参与下游信号通路，促进B细胞成熟和增殖。了解Btk的功能和定位对于开发针对免疫缺陷和相关疾病的靶向疗法至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Btk 抗体 (E-9) 参考文献:

  1. BTK抑制剂和不良反应机制的比较分析。  |  Estupiñán, HY., et al. 2021. Front Cell Dev Biol. 9: 630942. PMID: 33777941
  2. 临床前表征pirtobrutinib，一种高度选择性、非共价（可逆）BTK抑制剂。  |  Gomez, EB., et al. 2023. Blood. 142: 62-72. PMID: 36796019
  3. 在慢性淋巴细胞白血病中，继羟基布替尼之后，出现了共价BTK抑制剂。  |  Mato, AR., et al. 2023. N Engl J Med. 389: 33-44. PMID: 37407001
  4. BTK信号传导是慢性自发性荨麻疹病理生理学中的关键环节。  |  Bernstein, JA., et al. 2024. J Allergy Clin Immunol. 153: 1229-1240. PMID: 38141832
  5. 激酶受损的BTK突变对临床阶段的BTK和IKZF1/3降解剂NX-2127敏感。  |  Montoya, S., et al. 2024. Science. 383: eadi5798. PMID: 38301010
  6. BTK驱动中性粒细胞活化，从而产生杀菌抗真菌免疫。  |  Desai, JV., et al. 2024. J Clin Invest. 134: PMID: 38696257
  7. CD28 与人 Jurkat 白血病 T 细胞系中的 Tec 家族激酶 ITK/EMT 相关，并诱导其立即酪氨酸磷酸化和活化。  |  August, A., et al. 1994. Proc Natl Acad Sci U S A. 91: 9347-51. PMID: 7524075
  8. 鉴定在 T 细胞系中主要表达的新型蛋白酪氨酸激酶 Rlk。  |  Hu, Q., et al. 1995. J Biol Chem. 270: 1928-34. PMID: 7829530
  9. TXK是一种在T细胞中表达的新型人类酪氨酸激酶，与Tec家族激酶具有相同的序列，并映射到4p12。  |  Haire, RN., et al. 1994. Hum Mol Genet. 3: 897-901. PMID: 7951233
  10. 位于染色体 Xp22.2 的 BTK/ITK/TEC/TXK 家族的新型非受体酪氨酸激酶基因 BMX。  |  Tamagnone, L., et al. 1994. Oncogene. 9: 3683-8. PMID: 7970727
  11. X 连锁丙种球蛋白血症和 X 连锁免疫缺陷基因的共定位。  |  Thomas, JD., et al. 1993. Science. 261: 355-8. PMID: 8332900
  12. 造血细胞中新型蛋白酪氨酸激酶 Emb 和 Emt 的结构和表达。  |  Yamada, N., et al. 1993. Biochem Biophys Res Commun. 192: 231-40. PMID: 8476425

订购信息

产品名称产品编号规格价格数量收藏夹

Btk 抗体 (E-9)

sc-28387
200 µg/ml
$316.00

Btk (E-9): m-IgG Fc BP-HRP 套装

sc-551156
200 µg Ab; 10 µg BP
$354.00

Btk (E-9): m-IgGλ BP-HRP 套装

sc-551155
200 µg Ab; 40 µg BP
$354.00

Btk 抗体 (E-9) AC

sc-28387 AC
500 µg/ml, 25% agarose
$416.00

Btk 抗体 (E-9) HRP

sc-28387 HRP
200 µg/ml
$316.00

Btk 抗体 (E-9) FITC

sc-28387 FITC
200 µg/ml
$330.00

Btk 抗体 (E-9) PE

sc-28387 PE
200 µg/ml
$343.00

Btk 抗体 (E-9) Alexa Fluor® 488

sc-28387 AF488
200 µg/ml
$357.00

Btk 抗体 (E-9) Alexa Fluor® 546

sc-28387 AF546
200 µg/ml
$357.00

Btk 抗体 (E-9) Alexa Fluor® 594

sc-28387 AF594
200 µg/ml
$357.00

Btk 抗体 (E-9) Alexa Fluor® 647

sc-28387 AF647
200 µg/ml
$357.00

Btk 抗体 (E-9) Alexa Fluor® 680

sc-28387 AF680
200 µg/ml
$357.00

Btk 抗体 (E-9) Alexa Fluor® 790

sc-28387 AF790
200 µg/ml
$357.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Btk Antibody (E-9): sc-28387?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-16

I would like to use Btk Antibody (E-9): sc-28387 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-28387 is available conjugated to agarose, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-13

Is Btk Antibody(E-9) specific for Btk and no for Itk?

Asked by: addiagnost
Thank you for your question. Btk Antibody (E-9) is a mouse monoclonal that is specific for Btk. It is not expected to cross-react with Itk (also known as Emt, or IL2-inducible T-cell kinase).
Answered by: Technical Support
Date published: 2017-01-11
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Rated 5 out of 5 by from good productThis antibody works very well for our WB experiment.
Date published: 2018-08-22
Rated 5 out of 5 by from Good antibody for WBI used it on my human-immunoprecipitate samples, I got nice result, very good antibody for WB, with BSA as blocking, 1/1000 df
Date published: 2017-03-20
Rated 5 out of 5 by from Great for WBVery nice product for western blot applications !!
Date published: 2017-01-15
Rated 5 out of 5 by from Good for Western blotGreat bands seen in Western Blot with human and mouse samples.
Date published: 2016-12-23
Rated 5 out of 5 by from Worked greatWorked great. Got strong signal in WB.
Date published: 2015-10-06
Rated 4 out of 5 by from Publishable data in IP using human DeFewPublishable data in IP using human DeFew (human B lymphoma) cells -SCBT Publication Review
Date published: 2015-06-28
Rated 4 out of 5 by from Robust bands seen in WB using MEGRobust bands seen in WB using MEG-01, NAMALWA, BJAB and U-698-M whole cell lysates. -SCBT QC
Date published: 2015-04-28
Rated 4 out of 5 by from Various publishable data in WB using humanVarious publishable data in WB using human K562 and PBMCs. -SCBT Publication Review
Date published: 2015-02-12
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Btk 抗体 (E-9) is rated 4.6 out of 5 by 9.
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