Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BTEB2 Double Nickase Plasmid (m): sc-419372-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BTEB2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BTEB2 Double Nickase Plasmid (m) and BTEB2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Klf5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BTEB2 Antibody (G-7): sc-398470
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BTEB2 Double Nickase Plasmid (m) | sc-419372-NIC | 20 µg | $410.00 | |||
BTEB2 Double Nickase Plasmid (m2) | sc-419372-NIC-2 | 20 µg | $410.00 |
Mouse Klf5 encodes the transcription factor BTEB2 (Krüppel-like factor 5), a zinc-finger DNA-binding protein that regulates proliferative and differentiation programs in epithelial and mesenchymal lineages. BTEB2 integrates signals from MAPK/ERK, Wnt/β-catenin, TGF-β, and PI3K/AKT pathways to control cell-cycle progression, migration, and extracellular matrix remodeling. In development and tissue homeostasis, Klf5 activity influences intestinal crypt dynamics, vascular and smooth muscle phenotypes, and barrier-forming epithelia through context-dependent transcriptional networks. Dysregulated Klf5/BTEB2 expression has been linked to inflammatory remodeling, fibrosis, and oncogenic transcriptional states, making it a useful node for mechanistic studies of growth control and lineage plasticity.
BTEB2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Klf5 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Klf5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Klf5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Klf5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.