Date published: 2026-6-4

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BSA Antibody (A23-A/D3): sc-65701

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Datasheets
  • BSA Antibody (A23-A/D3) is a mouse monoclonal IgG2a, cited in 1 publications, provided at 200 µg/ml
  • raised against purified bovine serum albumin
  • recommended for detection of Bovine Serum Albumin of bovine origin by WB, IP and IF
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for BSA Antibody (A23-A/D3). This work is in progress.
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BSA Antibody (A23-A/D3) is a mouse monoclonal IgG2a antibody that detects bovine serum albumin (BSA) in bovine samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). BSA is a crucial plasma protein in cows, playing a vital role in maintaining osmotic pressure within blood plasma, which is essential for the proper distribution of body fluids between intravascular compartments and surrounding tissues. BSA′s structure consists of a single polypeptide chain that forms a heart-shaped conformation, allowing BSA to effectively bind various ligands, including fatty acids, hormones, and drugs. This unique structure not only contributes to BSA′s solubility and stability but also facilitates BSA′s function as a carrier protein, enabling the transport of these molecules throughout the bloodstream. BSA′s high availability and excellent solubility characteristics make BSA an ideal reagent for laboratories developing immunoassays, while BSA′s numerous functional groups provide ample opportunities for conjugation with other compounds, enhancing BSA′s utility in various experimental applications. Anti-BSA antibody (A23-A/D3) is therefore an invaluable tool for researchers working with bovine samples, ensuring accurate detection and analysis of this essential protein.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

BSA Antibody (A23-A/D3) References:

  1. Interaction of different polyphenols with bovine serum albumin (BSA) and human salivary alpha-amylase (HSA) by fluorescence quenching.  |  Soares, S., et al. 2007. J Agric Food Chem. 55: 6726-35. PMID: 17636939
  2. Investigation on interaction and sonodynamic damage of fluorescein derivants to bovine serum albumin (BSA) under ultrasonic irradiation.  |  Zou, M., et al. 2013. Spectrochim Acta A Mol Biomol Spectrosc. 110: 364-76. PMID: 23583854
  3. Spectroscopic study on the interaction between mononaphthalimide spermidine (MINS) and bovine serum albumin (BSA).  |  Tian, Z., et al. 2015. J Photochem Photobiol B. 142: 103-9. PMID: 25528194
  4. Spectroscopic Study on the Interaction between Naphthalimide-Polyamine Conjugates and Bovine Serum Albumin (BSA).  |  Tian, ZY., et al. 2015. Molecules. 20: 16491-523. PMID: 26378511
  5. Effect of apatite formation of biphasic calcium phosphate ceramic (BCP) on osteoblastogenesis using simulated body fluid (SBF) with or without bovine serum albumin (BSA).  |  Huang, L., et al. 2017. Mater Sci Eng C Mater Biol Appl. 70: 955-961. PMID: 27772726
  6. Monitoring the protective ability of thymoquinone mixture with p-cymene against bovine serum albumin (BSA) glycation: MCR-ALS analysis based on combined spectroscopic and electrochemical methods.  |  Benvidi, A., et al. 2018. Int J Biol Macromol. 107: 2465-2474. PMID: 29074084
  7. Molecular insight on the binding of monascin to bovine serum albumin (BSA) and its effect on antioxidant characteristics of monascin.  |  Wu, S., et al. 2020. Food Chem. 315: 126228. PMID: 31991257
  8. ROS-Targeted Depression Therapy via BSA-Incubated Ceria Nanoclusters.  |  Fu, S., et al. 2022. Nano Lett. 22: 4519-4527. PMID: 35583518
  9. In vitro characteristics of X- and Y-bearing ram spermatozoa sorted by bovine serum albumin (BSA) column and TLR7/8 ligand R848.  |  Yotov, S., et al. 2024. Anim Reprod Sci. 263: 107450. PMID: 38461673
  10. Interactions of three berberine mid-chain fatty acid salts with bovine serum albumin (BSA): Spectroscopic analysis and molecular docking.  |  Fan, S., et al. 2024. Int J Biol Macromol. 274: 133370. PMID: 38917913

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

BSA Antibody (A23-A/D3)

sc-65701
200 µg/ml
$322.00

I am using BSA (A23-A/D3): sc-65701 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jerojero
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Nice antibody for BSA detectionWe used this antibody in a western blot experiment to detect Bovine Serum Albumin and got a perfect band at the correct molecular weight
Date published: 2017-01-30
Rated 5 out of 5 by from Robust band seen in WB using BSARobust band seen in WB using BSA. -SCBT QC
Date published: 2014-02-10
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BSA Antibody (A23-A/D3) is rated 5.0 out of 5 by 2.
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