Date published: 2026-4-5

1-800-457-3801

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Broad Range Markers: sc-2361

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Datasheets
  • Recommended use is 1-5 µl per lane in SDS-PAGE
  • Each vial contains 500 µl volume, sufficient for 100-500 uses
  • The ladder consists of eight bands (unstained molecular weights). Unstained standard sizes: 200 kDa, 97 kDa, 66 kDa, 44 kDa, 29 kDa, 17 kDa, 14 kDa, 6 kDa
  • Broad Range Markers are provided ready to use in SDS-PAGE loading buffer and may be loaded directly into an SDS-PAGE gel. No dilution or reconstitution is required
  • Prestained Broad Range Markers are SDS-PAGE protein standards useful in gel electrophoresis and Western blotting applications
  • Prestained markers can be visualized during SDS-PAGE electrophoresis gel running and serve as a guide for ensuring accurate gel running time
  • Prestained Broad Range Markers are also valuable controls for verifying transfer efficiency of proteins
  • Broad Range Markers are recommended for approximating the molecular weight of target proteins in Western blotting applications
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Broad Range Markers References:

  1. The Arabidopsis homolog of yeast TAP42 and mammalian alpha4 binds to the catalytic subunit of protein phosphatase 2A and is induced by chilling.  |  Harris, DM., et al. 1999. Plant Physiol. 121: 609-17. PMID: 10517853
  2. The splicing factor, Prp40, binds the phosphorylated carboxyl-terminal domain of RNA polymerase II.  |  Morris, DP. and Greenleaf, AL. 2000. J Biol Chem. 275: 39935-43. PMID: 10978320
  3. An improved formulation of SYPRO Ruby protein gel stain: comparison with the original formulation and with a ruthenium II tris (bathophenanthroline disulfonate) formulation.  |  Berggren, KN., et al. 2002. Proteomics. 2: 486-98. PMID: 11987123
  4. Alpha(1)-antitrypsin and antichymotrypsin in human milk: origin, concentrations, and stability.  |  Chowanadisai, W. and Lönnerdal, B. 2002. Am J Clin Nutr. 76: 828-33. PMID: 12324297
  5. Cloning and bacterial expression of postnatal mouse heart FGF-16.  |  Sontag, DP. and Cattini, PA. 2003. Mol Cell Biochem. 242: 65-70. PMID: 12619867
  6. Extended in vitro culture of Microplitis croceipes teratocytes and secretion of TSP14 protein.  |  Hoy, HL. and Dahlman, DL. 2002. J Insect Physiol. 48: 401-409. PMID: 12770089
  7. Optimized conditions for diluting and reusing a fluorescent protein gel stain.  |  Ahnert, N., et al. 2004. Electrophoresis. 25: 2506-10. PMID: 15300769
  8. Characterization of a new Bacillus subtilis peptidoglycan hydrolase gene, yvcE (named cwlO), and the enzymatic properties of its encoded protein.  |  Yamaguchi, H., et al. 2004. J Biosci Bioeng. 98: 174-81. PMID: 16233686
  9. PilJ localizes to cell poles and is required for type IV pilus extension in Pseudomonas aeruginosa.  |  DeLange, PA., et al. 2007. Curr Microbiol. 55: 389-95. PMID: 17713814
  10. Sex steroids effects on the content of GAD, TH, GABA(A), and glutamate receptors in the olfactory bulb of the male rat.  |  Guerra-Araiza, C., et al. 2008. Neurochem Res. 33: 1568-73. PMID: 18363096
  11. Conjugates of bovine serum albumin with chitin oligosaccharides prepared through the Maillard reaction.  |  Ledesma-Osuna, AI., et al. 2010. J Agric Food Chem. 58: 12000-5. PMID: 21043451
  12. The main virulence determinant of Yersinia entomophaga MH96 is a broad-host-range toxin complex active against insects.  |  Hurst, MR., et al. 2011. J Bacteriol. 193: 1966-80. PMID: 21278295
  13. Full length and protease domain activity of chikungunya virus nsP2 differ from other alphavirus nsP2 proteases in recognition of small peptide substrates.  |  Saisawang, C., et al. 2015. Biosci Rep. 35: PMID: 26182358
  14. Expression of a functional thyroglobulin fragment in a baculovirus system.  |  den Hartog, MT., et al. 1993. J Mol Endocrinol. 11: 161-6. PMID: 8297472
  15. Antiproliferative effects of yogurt fractions obtained by membrane dialysis on cultured mammalian intestinal cells.  |  Ganjam, LS., et al. 1997. J Dairy Sci. 80: 2325-9. PMID: 9361204

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Broad Range Markers

sc-2361
500 µl
$78.00

I have to boil the marker before use? I did not boil at the first time, but the bands did not see completely separated.

Asked by: Taniaght
Thank you for your question. Please contact technical service (800)-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2023-07-25

Will this marker show up during the imaging step of the WB? Or is it only visualized when running the SDS-page?

Asked by: MiracleV
Thank you for your question. These will only show up during the SDS-Page however we do have other products that will show up on the final image of the western blot. Please call Technical Service for more information on our Cruz Markers and our MW Tag Antibodies. -Tech Service
Answered by: Technical Service 3
Date published: 2021-01-21

Hi I am about to use your broad range prestained marker protein standards (sc-2361) on a gel for the first time and wanted to ask a few questions: 1) other than thawing the standard, does it require boiling for 2 min ? (2) does it contain 4 M urea ?

Asked by: MichaelH
Thank you for your question. Yes, we recommend boiling the marker before use. You can see the recommended protocol here: https://datasheets.scbt.com/sc-2361.pdf It does not contain Urea.
Answered by: Tech Service
Date published: 2022-03-17

is it possible to take a trial of this product? Even 3micro liter would be nice.

Asked by: TheHeroZeus
Thank you for your question. We do not currently offer sample sizes for our Broad Range Markers: sc-2361. If you purchase the product, we do warranty the performance of the product.
Answered by: Technical Support
Date published: 2017-10-30
  • y_2026, m_3, d_26, h_7CST
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Rated 4 out of 5 by from Very Useful product!!!This is a very useful product in our lab activities..will recommend this to others
Date published: 2024-09-17
Rated 4 out of 5 by from Grest signalI use this prestained marker to detect the gel run status. It works properly and Is sufficient a low ammount. I use 2,5 ul in 15 well gels
Date published: 2022-06-06
Rated 5 out of 5 by from Great PerformanceThese marker do a great Job for us in daily lab routines
Date published: 2018-09-19
Rated 5 out of 5 by from A satisfying productSome time ago,our laboratory purchased the Broad Range Markers and yesterday because of the needs of the experiment I used it.To my surprise it is really a goood product.The Broad Range Markers is very convenient to use, and the experimental results are also very satisfactory.So our lab is ready to buy some more.If you need, I believe this is a good choice.
Date published: 2016-12-14
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Broad Range Markers is rated 4.5 out of 5 by 4.
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