Each vial contains 500 µl volume, sufficient for 100-500 uses
The ladder consists of eight bands (unstained molecular weights). Unstained standard sizes: 200 kDa, 97 kDa, 66 kDa, 44 kDa, 29 kDa, 17 kDa, 14 kDa, 6 kDa
Broad Range Markers are provided ready to use in SDS-PAGE loading buffer and may be loaded directly into an SDS-PAGE gel. No dilution or reconstitution is required
Prestained Broad Range Markers are SDS-PAGE protein standards useful in gel electrophoresis and Western blotting applications
Prestained markers can be visualized during SDS-PAGE electrophoresis gel running and serve as a guide for ensuring accurate gel running time
Prestained Broad Range Markers are also valuable controls for verifying transfer efficiency of proteins
Broad Range Markers are recommended for approximating the molecular weight of target proteins in Western blotting applications
I have to boil the marker before use? I did not boil at the first time, but the bands did not see completely separated.
Asked by: Taniaght
Thank you for your question. Please contact technical service (800)-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2023-07-25
Will this marker show up during the imaging step of the WB? Or is it only visualized when running the SDS-page?
Asked by: MiracleV
Thank you for your question.
These will only show up during the SDS-Page however we do have other products that will show up on the final image of the western blot. Please call Technical Service for more information on our Cruz Markers and our MW Tag Antibodies.
-Tech Service
Answered by: Technical Service 3
Date published: 2021-01-21
Hi I am about to use your broad range prestained marker protein standards (sc-2361) on a gel for the first time and wanted to ask a few questions: 1) other than thawing the standard, does it require boiling for 2 min ?
(2) does it contain 4 M urea ?
Asked by: MichaelH
Thank you for your question. Yes, we recommend boiling the marker before use. You can see the recommended protocol here:
https://datasheets.scbt.com/sc-2361.pdf
It does not contain Urea.
Answered by: Tech Service
Date published: 2022-03-17
is it possible to take a trial of this product? Even 3micro liter would be nice.
Asked by: TheHeroZeus
Thank you for your question. We do not currently offer sample sizes for our Broad Range Markers: sc-2361. If you purchase the product, we do warranty the performance of the product.
Rated 4 out of
5 by
john from
Very Useful product!!!This is a very useful product in our lab activities..will recommend this to others
Date published: 2024-09-17
Rated 4 out of
5 by
ginger5212 from
Grest signalI use this prestained marker to detect the gel run status. It works properly and Is sufficient a low ammount. I use 2,5 ul in 15 well gels
Date published: 2022-06-06
Rated 5 out of
5 by
SCG25 from
Great PerformanceThese marker do a great Job for us in daily lab routines
Date published: 2018-09-19
Rated 5 out of
5 by
Chen xiaobai from
A satisfying productSome time ago,our laboratory purchased the Broad Range Markers and yesterday because of the needs of the experiment I used it.To my surprise it is really a goood product.The Broad Range Markers is very convenient to use, and the experimental results are also very satisfactory.So our lab is ready to buy some more.If you need, I believe this is a good choice.